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Primary Culture of Mouse Dopaminergic Neurons
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JoVE Journal Neuroscience
Primary Culture of Mouse Dopaminergic Neurons

Primary Culture of Mouse Dopaminergic Neurons

DOI:
10.3791/51751-v

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11:58 min

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September 08, 2014

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, ,
1CNRS, UMR-5203,Institut de Génomique Fonctionnelle, Montpellier, 2Inserm,U661, Montpellier, 3Universités de Montpellier

Chapters

  • 00:05Title
  • 01:29Dissection of Mouse Mesencephalon
  • 04:40Cell Dissociation
  • 06:37Cell Plating
  • 07:29Immunofluorescence Protocol
  • 09:20Results: Primary Culture of Mouse Dopaminergic Neurons
  • 10:49Conclusion

Summary

Automatic Translation

Automatic Translation

Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.

Tags

Keywords: Primary CultureMouse Dopaminergic NeuronsParkinson’s DiseaseNigrostriatal NeuronsNeurodegenerationVentral MesencephalonTransgenic Mouse ModelsImmunofluorescenceQPCRSecond MessengersNeuronal Death/survival

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