Mrc5 Lung Fibroblasts
Mrc5 Lung Fibroblasts - Related Videos
11:43
Establishing Primary Adult Fibroblast Cultures From Rodents
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Cited by 305 •
2010
This article describes a protocol for isolation and maintenance of primary fibroblast cultures from skin and lung tissue of wild rodents.
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
05:54
Rapid Culturing of Primary Murine Melanocytes and Fibroblasts: Isolating Melanocytes and Fibroblasts from Whole Murine Skin Sample
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2023
This video describes the rapid method to isolate and culture the melanocytes and fibroblast simultaneously from the postnatal mouse pup’s skin. These cells can be maintained and manipulated in vitro to study skin cell biology, wound healing, and melanoma.
06:04
Murine Dermal Fibroblast Isolation by FACS
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Cited by 28 •
2016
Fibroblast behavior underlies a spectrum of clinical entities, but they remain poorly characterized, largely due to their inherent heterogeneity. Traditional fibroblast research relies upon in vitro manipulation, masking in vivo fibroblast behavior. We describe a FACS-based protocol for the isolation of mouse skin fibroblasts that does not require cell culture.
07:06
A Simple Method of Mouse Lung Intubation
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Cited by 50 •
2013
This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.
FAQs
Exposure to microbial or inflammatory stimuli can alter gene expression in these cells and promote release of mediators such as cytokines and chemokines. This response gives researchers a cellular readout of how pulmonary stromal cells participate in inflammatory signaling. Comparing mediator production under different stimuli can help connect a trigger with downstream changes relevant to lung inflammation.
Extracellular-matrix production preserves a central fibroblast function within the experimental model. It allows investigators to examine infection or inflammation in cells that retain a stromal role rather than focusing only on specialized immune cells. Changes observed alongside matrix-related activity can therefore help relate cellular responses to processes associated with pulmonary tissue structure and injury.
The culture can support investigations of several related but distinct outcomes, including viral entry, replication, host-cell gene-expression changes, mediator release, and infection-associated cytotoxicity. Examining these outcomes separately helps distinguish events caused by the presence or multiplication of a virus from responses generated by the host cell. That distinction improves interpretation of infection mechanisms in lung-derived cells.
Reproducible growth supports standardized comparisons between experimental conditions. Investigators can use comparable cultures to evaluate how different microbial or inflammatory stimuli influence cellular responses, antiviral activity, or cytotoxicity. Consistency is especially valuable when experiments connect molecular or mediator-level changes with broader questions about pulmonary inflammation and pathogen-induced tissue injury.
These cultures can be used to examine viral entry and replication, evaluate host-cell responses, and assess infection-associated cytotoxicity. They also provide a setting for studying antiviral activity under controlled in vitro conditions. Together, these applications allow researchers to investigate both pathogen behavior and the consequences of infection for human lung-derived stromal cells.
Their relevance comes from combining a human pulmonary stromal context with measurable responses to microbial or inflammatory stimuli. Cytokine and chemokine release can be studied alongside changes in gene expression, antiviral activity, and cytotoxicity. Findings from these experiments help relate cellular behavior to mechanisms of lung inflammation and tissue injury associated with infection.