Murine Bal Fluid
Murine Bal Fluid - Related Videos
11:47
Bronchoalveolar Lavage (BAL) for Research; Obtaining Adequate Sample Yield
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Cited by 47 •
2014
We describe a research technique for fiberoptic bronchoscopy and bronchoalveolar lavage using low pressure suction. The technique is used to harvest immune cells from the lung bronchoalveolar surfaces. Local anesthetic and mild conscious sedation (midazolam) is used. Subjects tolerate the procedure well and experience minimal side effects.
03:37
Visualization of Extracellular Traps in Macrophages from Human Bronchoalveolar Lavage Fluids
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2025
This video demonstrates the immunolabeling of macrophage extracellular traps, METs, in bronchoalveolar macrophages from bronchoalveolar lavage fluid, and their visualization using a confocal microscope.
09:58
A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice
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Cited by 10 •
2010
Repeated measurements of rodent respiratory physiology and sampling of airway inflammatory cells are desirable, but generally not feasible. Here we describe a repeatable method for orally intubating mice that permits repeated measurements of airway hyperreactivity and sampling of airway inflammatory cells.
05:12
Murine Nasal Lavage Fluid Collection without Blood Contamination
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2025
The present protocol describes a novel method for avoiding blood contamination in murine nasal lavage fluid (NLF). Since this method ensures the NLF collection without blood contamination, it allows for more accurate detections of immunological components and various respiratory pathogens.
07:03
Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
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Cited by 155 •
2017
The health status of the lung is reflected by the type and number of immune cells that are present in the bronchioles of the lung. We describe a bronchoalveolar lavage technique that allows the isolation and study of nonadherent cells and soluble factors from the lower respiratory tract of mice.
FAQs
Different BAL fluid constituents answer different experimental questions. Airway cells support cell counts and cytology, while soluble proteins and cytokines provide biochemical measures of inflammation. Microbial components can indicate infectious involvement, and changes in recovered material can help characterize pulmonary barrier injury. Combining these readouts gives a broader assessment than relying on a single measurement.
Cell counts estimate the extent of immune-cell recruitment into the airways, whereas cytology adds visual information about the recovered cells and sample composition. Together, these analyses help determine whether an experimental condition is associated with increased pulmonary inflammation and provide a cellular complement to protein, cytokine, or pathogen measurements.
Soluble proteins and cytokines provide molecular indicators of the pulmonary environment. Their measurement can help evaluate inflammatory activity and identify changes associated with barrier injury in the airways. These assays complement cellular observations by showing how tissue responses are reflected in the fluid recovered from the alveolar space during health, inflammation, or infection studies.
Collection begins by introducing sterile fluid through the mouse trachea and then withdrawing it from the lower airways. The recovered lavage contains material from the alveolar space, including cells, soluble proteins, cytokines, and microbial components. Researchers can then divide the sample for cell counts, cytology, protein or cytokine assays, and pathogen detection.
In infection research, pathogen detection and analysis of microbial components in the recovered fluid can be combined with cellular and inflammatory measurements. This approach helps relate the presence of infectious material to immune-cell recruitment, cytokine responses, and pulmonary barrier injury. The resulting profile can contribute to characterization of how respiratory tissues respond during infection.
Murine BAL fluid is useful when researchers need comparable measurements across experimental groups or time points. Cell, protein, cytokine, and pathogen analyses can help evaluate disease mechanisms, compare experimental treatments, and monitor respiratory responses over time. In immunology and infection studies, these outcomes connect local airway changes with the progression or modification of pulmonary inflammation.