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Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
DOI:

07:03 min

May 04, 2017

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Chapters

  • 00:05Title
  • 00:52Broncoalveolar Lavage (BAL)
  • 02:58BAL Fluid Component Collection and Immunostaining for Flow Cytometry (FACS)
  • 04:27Results: Representative BAL Immune Cell Detection
  • 06:17Conclusion

Summary

Automatic Translation

The health status of the lung is reflected by the type and number of immune cells that are present in the bronchioles of the lung. We describe a bronchoalveolar lavage technique that allows the isolation and study of nonadherent cells and soluble factors from the lower respiratory tract of mice.

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