Murine Lung Model
Murine Lung Model - Related Videos
10:01
Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation
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Cited by 20 •
2012
Obliterative bronchiolitis is the key impediment to the long-term survival of lung transplant recipients and the lack of a robust preclinical model precludes examining obliterative bronchiolitis immunopathogenesis. Unlike other solid organ transplants, vascularized mouse lung transplantation has only recently been developed. Here we show our independently developed obliterative bronchiolitis model after murine orthotopic single-lung transplantation.
03:06
Preparation of a Murine Oropharyngeal Aspiration Model to Establish Bacterial Lung Infections
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2026
Source: Nielsen, T. B., et al., Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia. J. Vis. Exp. (2018)This video demonstrates the procedure of delivering bacteria through the oropharynx into the lungs of an anesthetized mouse to induce lung infection and trigger an immune response.
05:17
Cardiopulmonary Complex Decellularization: A Technique for Decellularizing Heart and Lungs from Murine Model
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2025
This video demonstrates a method of producing decellularized extracellular matrix or ECM scaffolds by applying antegrade perfusion of detergents through the vasculature. The structural ECM scaffolds can then be used to analyze ECM remodeling associated with cardiopulmonary disease.
05:49
Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation
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Cited by 5 •
2023
Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.
05:54
An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs
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2025
This video demonstrates an in vitro technique to identify immune cells residing in murine lungs. After isolating target immune cells from murine lung tissue, they are surface-labeled using a cocktail of fluorophore-tagged antibodies against specific markers on innate and adaptive immune cells. Additionally, monocytes, macrophages, and dendritic cells are labeled using an intracellular marker. The various immune cell types are then identified using flow cytometry.
FAQs
Interpretation depends on examining how several defenses interact rather than measuring inflammation alone. Epithelial barriers can influence exposure and tissue injury, while alveolar macrophages and neutrophils contribute to early pulmonary responses and pathogen clearance. Lymphocytes and cytokine signaling add further regulation. Together, these components help explain why infection or inflammation produces particular tissue and fluid findings.
Cytine signaling helps coordinate communication among lung epithelial cells and immune populations during infection or inflammation. Its activity can be considered alongside macrophage, neutrophil, and lymphocyte responses to determine how the host balances pulmonary inflammation with pathogen clearance. Measuring this signaling in relation to tissue and fluid changes can clarify mechanisms underlying respiratory disease.
The outcome reflects the interaction between the introduced pathogen, antigen, or inflammatory stimulus and the host defenses present in the lung. Epithelial integrity, alveolar macrophage and neutrophil activity, lymphocyte responses, and cytokine signaling can all influence the balance. Studying these variables together allows investigators to distinguish protective clearance mechanisms from inflammatory changes associated with lung injury.
After introducing the selected pathogen, antigen, or inflammatory stimulus into the airways, researchers assess pulmonary responses through controlled sampling. Lung tissue provides information about local structural and cellular changes, bronchoalveolar lavage fluid reflects airway-associated findings, and blood offers a systemic comparison. Evaluating these materials together connects local lung inflammation with broader immune responses.
These models are useful when investigators need to examine protective immunity, pathogen clearance, or inflammatory changes in a living respiratory system. Vaccine studies can focus on host responses after infectious challenge, while therapeutic studies can assess effects on disease-associated inflammation or clearance. The approach also supports investigation of pneumonia, viral infection, tuberculosis, asthma, and acute lung injury.
The models reproduce selected features of human respiratory diseases while allowing controlled analysis of lung tissue, bronchoalveolar lavage fluid, and blood. Results can reveal host-pathogen interactions, immune mechanisms, and responses to candidate interventions. Because they reproduce selected rather than necessarily complete disease features, their main value is guiding hypotheses and identifying mechanisms for further human respiratory research.