Neutrophil Recruitment
Neutrophil Recruitment - Related Videos
04:29
Monitoring Neutrophil Recruitment Dynamics and Bacterial Burden at the Infection Site in a Murine Model
0 Views •
2025
This video demonstrates in vivo imaging to study the neutrophil recruitment dynamics at an infection site in an immunocompromised, transgenic mouse with GFP-expressing neutrophils. It involves injecting luminescent bacteria to cause an infection. This leads to neutrophil recruitment to the infection site, whose tracking is done by imaging.
05:52
An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment
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2025
The video demonstrates a method for investigating the impact of stromal cells on leukocyte recruitment. In a parallel plate flow chamber model, pro-inflammatory cytokines induce the expression of adhesion receptors by endothelial cells, facilitating neutrophil recruitment and migration. The cross-talk between stromal cells and endothelial cells diminishes neutrophil recruitment to the endothelial cells.
11:30
Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
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Cited by 28 •
2015
The ability of inflamed endothelium to recruit leukocytes from flow is regulated by mesenchymal stromal cells. We describe two in vitro models incorporating primary human cells that can be used to assess neutrophil recruitment from flow and examine the role that mesenchymal stromal cells play in regulating this process.
04:32
Induction and Visualization of Neutrophil Extracellular Traps in Pre-Labeled Neutrophils
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2025
This video demonstrates the induction and visualization of neutrophil extracellular traps or NETs in pre-labeled neutrophils using patient serum containing anti-neutrophil cytoplasmic antibodies. Furthermore, using an impermeable DNA dye and fluorescence microscopy enables the visualization of NETs.
07:12
Neutrophil Isolation Protocol
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Cited by 173 •
2008
Neutrophils are among the first cells to arrive on the site of inflammatory immune response, and their functions and mechanisms have been studied extensively in vitro. We demonstrate a standard density gradient separation method to isolate human neutrophils from whole blood using commercially available separation media.
FAQs
Selectins and integrins control different checkpoints in the vascular phase. Endothelial selectins first slow circulating neutrophils by promoting rolling. Chemokine signals then activate neutrophil integrins, enabling firm adhesion to the vessel wall. This division of labor lets recruitment remain sequential rather than random, so investigators can distinguish defects in initial capture from defects in stable attachment.
Chemokines provide directional information after adhesion has been initiated. Their gradients guide neutrophils away from the vessel and toward the infected or injured region, concentrating cells where antimicrobial activity is needed. The gradient therefore influences not only whether cells leave the bloodstream, but also how effectively they reach the relevant tissue location.
Diapedesis is the vessel-crossing stage that connects firm adhesion with tissue entry. After passing through the vessel wall, neutrophils move along local chemical gradients and can deploy phagocytosis, degranulation, and reactive oxygen species against microbes. Studying these stages separately helps link a trafficking defect to a later failure of antimicrobial defense.
Recruitment must be strong enough to contain infection but limited enough to preserve host tissue. When neutrophil accumulation is excessive or prolonged, the same antimicrobial activities that help eliminate microorganisms can contribute to tissue damage. This balance is central to immunology because disease severity may reflect both inadequate cellular arrival and an inflammatory response that does not resolve.
A practical interpretation starts with endothelial selectin-dependent rolling, followed by chemokine-driven integrin activation and firm adhesion. The analysis then considers diapedesis, movement along tissue gradients, and antimicrobial activity after arrival. Organizing observations in this order helps researchers determine whether an altered outcome originates in vascular capture, tissue entry, directional migration, or downstream neutrophil function.
Neutrophil recruitment links innate immune defense to broader inflammatory disease research. In infection studies, it helps explain how cells reach affected tissue and contribute to microbial containment. In inflammation and autoimmune disease, the same trafficking process provides a framework for examining harmful immune accumulation. It also identifies a biological process that therapeutic strategies may seek to regulate.