Pdms Device Preparation
Pdms Device Preparation - Related Videos
14:48
PDMS Device Fabrication and Surface Modification
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04:45
Non-plasma Bonding of PDMS for Inexpensive Fabrication of Microfluidic Devices
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In this video we demonstrate how to use the neuron microfluidic device without plasma bonding.
08:58
Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device
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Microfluidic double emulsions generation typically involves devices with patterned wettability or custom-fabricated glass components. Here we describe the fabrication and testing of an all polydimethylsiloxane (PDMS) double emulsion generator that does not require surface treatment or complicated fabrication processes, and is capable of producing double emulsions down to 14 µm.
09:41
Stretching Micropatterned Cells on a PDMS Membrane
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08:29
Thermal Measurement Techniques in Analytical Microfluidic Devices
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Here, we present three protocols for thermal measurements in microfluidic devices.
FAQs
Degassing removes bubbles from the PDMS mixture before it is placed over the patterned mold. This helps preserve the intended channel geometry and supports controlled movement of cells, pathogens, fluids, or chemical gradients. Removing bubbles is especially important when experiments depend on continuous microfluidic pathways or direct optical observation of biological activity inside the finished device.
After thermal curing, oxygen-plasma treatment activates the PDMS surface so it can bond to glass or to another PDMS layer. This bonding step closes the patterned channels and converts the molded layer into an enclosed microfluidic device. The bonding partner can therefore provide either a glass interface or an all-PDMS configuration for biological experiments.
Transparency provides direct optical access to activity inside the device, while flexibility supports fabrication of microfluidic structures for biological assays. Together with the ability to work with small sample volumes, these properties make PDMS devices practical for observing cells, pathogens, fluids, and gradients in controlled settings. Their value lies in linking microscale handling with visible experimental outcomes.
Thermal curing should precede oxygen-plasma treatment because the molded PDMS must first become the formed layer that will be bonded. Plasma activation is then applied to the cured surface, allowing attachment to glass or another PDMS layer. Keeping these stages in sequence links mold fidelity with channel enclosure and prepares the device for controlled biological experiments.
Microfluidic channels can provide controlled handling of cells and chemical gradients, allowing investigators to examine immune-cell movement under defined conditions. Because the device uses small sample volumes and offers direct optical access, researchers can follow migration within the channel while limiting the amount of biological material required. This makes the platform relevant to experiments on immune-cell behavior during infection.
They can support studies of host-pathogen interactions, barrier function, and infection dynamics. The same microscale format allows pathogens, host cells, fluids, and chemical gradients to be handled in controlled channels, while optical access supports direct observation. Consequently, investigators can use the platform to examine how infection-associated processes develop across cellular barriers and change during experimental observation.