Peritoneal Lavage
Peritoneal Lavage - Related Videos
04:59
Peritoneal Low-density Neutrophil Isolation: A Technique to Obtain Low-density Neutrophils from Peritoneal Lavage Fluid Using Magnetic Activated Cell Sorting
0 Views •
2023
In this video, we describe the procedure for isolating low-density neutrophils, or LDNs, from the peritoneal lavage fluid of a postoperative human patient. The LDNs thus obtained can be used in further downstream experiments to understand their role in tumor growth and progression.
05:05
In Vivo Activation of Peritoneal Macrophages in Response to Antibodies in a Murine Model
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2025
This video demonstrates the activation of a mouse's peritoneal macrophages using intravenous immunoglobulin and bacterial lipopolysaccharide. The co-stimulation activates the peritoneal macrophages into anti-inflammatory macrophages possessing immune regulatory activity.
04:17
A Technique to Isolate Primary Mast Cells from a Murine Peritoneal Cavity
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2025
This video demonstrates a peritoneal lavage method for isolating mature connective tissue-type mast cells from the peritoneal cavity of adult mice. Upon isolating a mixture of primary immune cells from the peritoneal cavity, selective proliferation of mast cells is performed under appropriate culture conditions.
11:47
Bronchoalveolar Lavage (BAL) for Research; Obtaining Adequate Sample Yield
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Cited by 47 •
2014
We describe a research technique for fiberoptic bronchoscopy and bronchoalveolar lavage using low pressure suction. The technique is used to harvest immune cells from the lung bronchoalveolar surfaces. Local anesthetic and mild conscious sedation (midazolam) is used. Subjects tolerate the procedure well and experience minimal side effects.
12:27
The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice
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Cited by 15 •
2014
The host immune response to pathogen infection is a tightly regulated process. Utilizing a lipopolysaccharide lung exposure model in mice, it is possible to conduct high resolution evaluations of the complex mechanisms associated with disease pathogenesis.
FAQs
Fluid movement across the serosal surfaces collects biological material distributed within the peritoneal cavity. The recovered material can therefore contain cells, microorganisms, and other components associated with local tissue injury or inflammation. Examining this combined sample gives researchers access to local events that may not be represented by observations from a single tissue site.
Lavaged cells can be examined to characterize the local host response, including leukocyte recruitment into the peritoneal cavity. Their recovery provides evidence that immune cells have accumulated at the site of inflammation or infection. This makes the technique useful for studying how local cellular responses relate to peritonitis, abdominal infection, and experimental immune mechanisms.
Different analyses answer different biological questions. Microscopy can examine recovered material directly, culture can investigate microorganisms, cytological analysis can characterize cells, and immunological assays can assess immune-related features. Using these approaches together supports a broader interpretation of whether the sample reflects inflammation, infection, tissue injury, or a combination of these processes.
The recovered sample supports parallel assessment of microorganisms and host-associated findings. Culture or microscopy can provide information about pathogen presence, while cytological and immunological analyses characterize cellular and immune responses. Considering these results together helps connect evidence of abdominal infection with the accompanying local inflammation rather than examining either the pathogen or the host response in isolation.
The core workflow consists of introducing sterile fluid into the peritoneal cavity, allowing it to wash the serosal surfaces, recovering the fluid, and analyzing the resulting sample. The recovered material may then undergo microscopy, culture, cytological analysis, or immunological assays. This sequence links sample collection directly to characterization of local infection, inflammation, or injury.
Researchers apply the technique when they need information about events occurring within the peritoneal cavity. Relevant settings include studies of peritonitis, abdominal infection, tissue injury, and experimental immune mechanisms. Because the recovered fluid contains material from the local environment, it can support investigation of pathogen presence and host responses and may contribute to diagnostic assessment.