Plant Resistance Screening
Plant Resistance Screening - Related Videos
08:17
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
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Cited by 22 •
2013
Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.
02:30
Seedling Flood Assay for Screening Bacterial Resistance in Tomatoes
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2025
Source: Hassan, J. A., et al. High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay. J. Vis. Exp. (2020).This video demonstrates a method to screen for bacterial resistance in different tomato strains using ten-day-old seedlings inoculated with a bacterial suspension. It explains how bacteria invade through stomata, deliver effector proteins to suppress plant immunity, and how plant responses differ between susceptible and resistant...
12:43
Electroantennographic Bioassay as a Screening Tool for Host Plant Volatiles
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Cited by 14 •
2012
A method to rapidly screen host plant volatiles by measurement of the electrophysiological response of adult navel orangeworm (Amyelois transitella) antennae to single components and blends via electroantennographic analysis is demonstrated.
10:22
Transmitting Plant Viruses Using Whiteflies
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Cited by 41 •
2013
Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...
12:47
A Technique to Screen American Beech for Resistance to the Beech Scale Insect (Cryptococcus fagisuga Lind.)
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Cited by 8 •
2014
Beech bark disease is initiated by feeding activities of the beech scale insect that create fungal entry points in the bark. Trees that are resistant to the scale insect are also disease resistant. Here we present the protocol we have developed to screen individual beech trees for beech scale resistance.
FAQs
Immune recognition allows a plant to detect infection-related threats, while signaling coordinates downstream defense responses. These processes can activate physical or chemical defenses that restrict pathogen colonization. Screening outcomes therefore reflect more than visible symptoms: differences among genotypes may indicate variation in recognition, signal transmission, or the effectiveness of defenses that limit pathogen growth.
Symptoms, lesion development, and pathogen growth describe different aspects of infection. A genotype may show limited visible damage while still supporting pathogen growth, or it may restrict growth with effects that are evaluated through symptoms. Considering multiple outcomes gives researchers a more complete basis for comparing resistance and identifying traits associated with effective defense.
When genotypes experience comparable infection assays, differences in symptom severity, lesion development, or pathogen growth can expose variation in their defensive capacity. Such comparisons help connect observable outcomes with underlying immune recognition, signaling, and physical or chemical defenses. The resulting patterns can identify genotypes or traits that warrant further study in plant immunity and resistance research.
A controlled assay begins by selecting plant genotypes and exposing them to a pathogen through inoculation. Researchers then assess outcomes such as symptom severity, lesion development, or pathogen growth. Comparing these measurements across genotypes provides evidence about how effectively each plant limits infection and creates a consistent foundation for studying resistance-related traits.
The method is useful when researchers need to identify plant traits linked to effective defense and select promising material for resistance breeding. By comparing infection outcomes among genotypes, investigators can distinguish plants that better limit disease or pathogen growth. These findings support the development of more resilient crops and contribute to strategies for improving crop health and food security.
In an immunology and infection context, screening links host responses with the outcomes of pathogen exposure. Comparing plants challenged by diverse pathogens can reveal how immune recognition, signaling, and physical or chemical defenses vary across host-pathogen interactions. This information supports host-pathogen studies by showing which defensive traits are associated with restricted colonization or reduced disease.