Skin Infection Model
Skin Infection Model - Related Videos
02:13
Generating an Ex Vivo Ovine Wounded Skin Model Infected with a Bacterial Pathogen
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2025
The video demonstrates the generation of an ex vivo ovine skin model infected with Staphylococcus aureus. This entails treating a lamb's forehead skin biopsy section with antibiotics, establishing a wound bed, positioning it on a permeable membrane in a multiwell plate with antibiotic-free medium, and establishing bacterial infection in the skin wound.
02:24
Developing a Mouse Model of Chronic Pseudomonas aeruginosa Skin Wound Infection
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2026
Source: de Vries, C. R., et.al. A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection. J. Vis. Exp. (2020)This video demonstrates the delayed inoculation of luminescent Pseudomonas aeruginosa into a mouse bearing two full-thickness dorsal skin wounds covered by a transparent film dressing. This facilitates biofilm formation and the establishment of chronic infection. Regular bioluminescence monitoring enables quantification of bacterial burden and tracking of infection...
03:25
Cultivation of Pathogenic Spirochetes to Investigate Skin Infection
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2026
Source: Berthold, A., et al. Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia. J. Vis. Exp.(2022)This video demonstrates the cultivation of pathogenic spirochetes from infected skin tissue to model dermal infection. It highlights their localization in the dermis, interaction with the extracellular matrix, and survival during selective culture conditions.
04:00
A Murine Skin Wound Infection Model to Study the Immune Response against a Bacterial Pathogen
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2025
This video demonstrates a technique to generate a murine model of skin wound infection. Upon infecting mice — engineered to express a fluorescent protein in neutrophils — with a genetically modified bioluminescent Staphylococcus aureus — a pathogenic bacteria. The bacterial burden at the infection site and the neutrophil recruitment dynamics can be studied using whole animal imaging.
01:29
Staphylococcal Skin Infections
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2026
Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
FAQs
It allows researchers to examine whether infection is associated with barrier disruption while simultaneously tracking microbial growth and host responses. These measurements connect physical damage in skin with inflammatory signaling and immune-cell recruitment. Comparing these outcomes across conditions helps clarify how epithelial defenses and innate immunity contribute to limiting infection.
These platforms represent different levels of skin organization. Cultured keratinocytes provide an epithelial setting, reconstructed tissue adds a more skin-like structure, and animal skin supplies an intact biological context. The choice determines which features of barrier disruption, microbial growth, inflammatory signaling, or immune-cell recruitment can be examined under controlled experimental conditions.
Key measurements include changes in the skin barrier, the amount or pattern of microbial growth, inflammatory signaling, and recruitment of immune cells. Together, these readouts show both tissue-level damage and defense activity. This combination is useful because a treatment or pathogen may affect microbial growth and host inflammation differently.
Controlled conditions make it easier to attribute differences in infection outcomes to the selected pathogen or intervention rather than to uncontrolled variation in the experimental system. Researchers can then compare barrier disruption, microbial growth, inflammatory signaling, and immune-cell recruitment across treatments or infection settings with greater reproducibility.
A typical workflow begins by selecting a skin platform, such as cultured keratinocytes, reconstructed tissue, or animal skin, and pairing it with a selected pathogen. Researchers then assess infection through barrier disruption, microbial growth, inflammatory signaling, and immune-cell recruitment. The resulting measurements support comparisons among pathogens, treatments, or therapeutic strategies.
Selection depends on which aspect of infection the study needs to reproduce and measure. Cultured keratinocytes, reconstructed skin tissue, and animal skin offer progressively different experimental contexts for examining host defense and microbial effects. Researchers can match the platform to the desired analysis while maintaining conditions suitable for reproducible comparisons.
They are useful when researchers need controlled evidence about how an intervention affects infection-related outcomes before clinical testing. Measurements of microbial growth can indicate antimicrobial activity, while barrier disruption, inflammatory signaling, and immune-cell recruitment reveal effects on tissue damage and host defense. The same framework can also support studies of pathogen virulence mechanisms.