Streptavidin Fitc Conjugate
Streptavidin Fitc Conjugate - Related Videos
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
0 Views •
2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
03:41
Analyzing Influenza Virus Internalization Using a Streptavidin Blocking Assay
0 Views •
2026
Source: Pohl, M. O. & Stertz, S. Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry. J. Vis. Exp. (2015)This video demonstrates the use of a streptavidin blocking assay to distinguish surface-bound from internalized influenza virus in human lung epithelial cells. Comparing fluorescence signals shows that a higher intensity after incubation indicates successful viral internalization.
02:56
FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans
0 Views •
2023
This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.
02:50
Conjugated Proteins
0 Views •
2020
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties. Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
01:19
Conjugation
0 Views •
2025
Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...
FAQs
Its exceptionally strong affinity creates a stable link between a biotinylated antibody, probe, or other recognition molecule and the fluorescent label. This separates target recognition from signal generation, allowing investigators to choose suitable biotinylated reagents while using the same detection conjugate. The arrangement supports flexible assay design for detecting cells, antigens, antibodies, and infectious agents.
FITC provides the optical component of the conjugate. After the streptavidin portion binds a biotinylated molecule associated with the target, FITC produces a green fluorescence signal when exposed to appropriate illumination. The resulting fluorescence marks where the binding event occurred and can be assessed through microscopy, flow cytometry, or plate-based assay formats.
The conjugate links a fluorescent reporter to a biotin-dependent detection arrangement rather than requiring the primary recognition molecule itself to carry FITC. This design can increase sensitivity and allows the same fluorescent reagent to work with different biotinylated antibodies or probes. Consequently, investigators can adapt detection schemes while preserving a common fluorescence-based readout.
A meaningful result depends on a biotinylated recognition molecule connecting the conjugate to the intended target and on illumination suitable for FITC fluorescence. The selected assay format also affects how the signal is observed, whether as cellular localization by microscopy, measured cell-associated fluorescence by flow cytometry, or a fluorescence response in a plate-based assay.
A typical workflow begins with a biotinylated antibody, probe, or related molecule that recognizes the target. The conjugate is then used to bind that biotin-associated recognition reagent, creating a fluorescent detection complex. Appropriate illumination and the selected readout platform, such as microscopy, flow cytometry, or a plate-based assay, are used to observe the resulting signal.
The conjugate can support assays that identify cells, antigens, antibodies, or infectious agents. In immunology and infection research, these readouts help examine host-pathogen interactions and evaluate immune responses. Its compatibility with indirect immunofluorescence, flow cytometry, microscopy, and plate-based formats lets researchers select a detection approach suited to the biological question.
Researchers can attach biotin to antibodies or probes directed toward a cellular, antigenic, or infectious target, then use the conjugate to generate a fluorescence signal when that recognition event occurs. Observing the signal in a chosen assay format provides evidence for target detection, supporting investigations of host-pathogen interactions and assessments of immune responses.