Cell Culture Transfection
Cell Culture Transfection - Related Videos
02:29
Antibody Production from a Batch Overgrowth Culture of Transfected Mammalian Cells
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2025
The video demonstrates a method for producing therapeutic antibodies using stably transfected mammalian cells. Tryptone supplementation stimulates cell division, resulting in overgrowth and supporting a higher antibody yield. The antibody-containing medium is harvested, filtered, and stored for further use.
07:40
Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
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Cited by 9 •
2015
This protocol describes a cell transplantation system using genetically modified, injectable spheroids. Cell spheroids are cultured on micropatterned culture plates and recovered after gene introduction using polyplex nanomicelles. This system facilitates prolonged transgene expression from the transplanted cells in host animals while maintaining the innate function of the cells.
08:51
Culture and Transfection of Zebrafish Primary Cells
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Cited by 3 •
2018
We present an efficient and easy-to-use protocol for preparing primary cell cultures of zebrafish embryos for transfection and live cell imaging as well as a protocol to prepare primary cells from adult zebrafish brain.
10:24
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
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Cited by 26 •
2011
Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.
07:28
An Introduction to Transfection
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2023
Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA into a cell enables the expression, or production, of proteins using the cells own machinery, whereas insertion of RNA into a cell is used to down-regulate the production of a specific protein by stopping translation. While the site of action for transfected RNA is the cytoplasm, DNA must be transported to the nucleus for effective transfection. There, the...
FAQs
Lipid-based carriers and electroporation represent different ways to help nucleic acids cross the cell membrane. The choice is tied to the experimental design rather than a single universal procedure. That decision can support different objectives, including temporary protein production, gene silencing, or genome modification, depending on the reagent and the intended biological outcome.
The intended outcome depends on the reagent and experimental design. Those choices can support temporary protein production, gene silencing, or genome modification, so the approach should match the biological question. Distinguishing these outcomes is important when interpreting altered gene expression, because protein production, silencing, and genome modification represent different experimental effects.
Crossing the cell membrane is the key enabling step because nucleic acids must reach cultured cells in a form that can alter gene expression. Lipid-based carriers and electroporation address this barrier through distinct approaches. Successful delivery connects the selected nucleic acid with the intended readout, such as protein production, gene silencing, or genome modification.
An experiment should match the nucleic acid and delivery approach to its intended effect. DNA, RNA, or other nucleic acids can be paired with lipid-based carriers or electroporation, while the reagent and design determine whether the study targets protein production, gene silencing, or genome modification. This alignment keeps the manipulation relevant to the biological question.
The method supports controlled studies of host-pathogen interactions by changing gene expression and examining cellular responses to pathogen-associated genes. The same framework can investigate immune signaling pathways and cytokine responses. These experiments connect a defined nucleic-acid manipulation with cellular behavior, helping researchers examine how genetic changes influence infection-related immune processes.
Cell culture transfection enables controlled testing of antiviral targets and reporter systems, linking selected nucleic-acid manipulations with cellular responses. In infection research, it can also support studies of genetic mechanisms that shape cellular susceptibility or resistance to infection. This combination connects molecular intervention with questions about antiviral activity and host defense.