Thermanox Coverslips
Thermanox Coverslips - Related Videos
02:41
B Cell Activation on an Antigen-Coated Coverslip
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2025
This video demonstrates B cell activation in vitro. The process involves antigen interaction with B cell receptors, leading to cytoskeleton remodeling, immune synapse formation, lysosome movement, antigen internalization, processing, and subsequent presentation on MHC molecules for B cell activation.
07:56
Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses
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Cited by 28 •
2016
Age-related increases in eye lens stiffness are linked to presbyopia. This protocol describes a simple, cost-effective method for measuring mouse lens stiffness. Mouse lenses, like human lenses, become stiffer with age. This method is precise and can be adapted for lenses from larger animals.
03:58
Implantation of Cranial Imaging Window in Mouse Model: A Surgical Procedure to Implant Glass-based Coverslip for Stable Optical Access to Regions of Murine Brain
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2023
This video presents a surgical procedure to implant cranial window in a mouse model for stable optical access to different regions of the brain.
02:44
Phase-Contrast Microscopy for Evaluating Developmental Mutants in Streptomyces
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2025
Source: Bennett, J. A. et al. Visual and Microscopic Evaluation of Streptomyces Developmental Mutants. J. Vis. Exp. (2018)This video demonstrates the use of phase-contrast microscopy to examine phenotypic differences between wild-type and mutant Streptomyces coelicolor. It outlines the steps involved in preparing and imaging aerial filaments to detect developmental defects in spore formation.
04:13
Co-Culturing Glioblastoma Cells on Patterned Neurons: A Technique to Monitor the Migration of Glioblastoma Cells on Rat Hippocampal Neurons In Vitro
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2025
This video describes a co-culture assay of glioblastoma cells on micropatterned neurons, which mimic the myelinated neuronal tracts invasion by the glioblastoma stem-like cells as they migrate on neurons in the peritumoral spaces. This assay can help analyze the effects of pharmacological drugs that inhibit glioblastoma cells' migration on neurons in a glioma.
FAQs
The tissue-culture treatment changes how the polystyrene surface interacts with the culture environment, improving wettability and cell attachment. Better attachment helps adherent cells spread across the coverslip rather than detach or remain poorly positioned. This creates a more consistent cellular substrate for later fixation, immunostaining, and microscopic examination.
Cell positioning matters because fixation and staining preserve and reveal structures where they are located. Cells that remain attached can be examined for morphology, while immunostaining can show the localization of selected proteins. In infection experiments, that spatial information helps relate microbial exposure to changes within individual host or immune cells.
Compared with a surface lacking the described tissue-culture treatment, the treated polystyrene substrate provides improved wettability and cell attachment. The practical consequence is not simply cell growth, but retention of cells in a usable arrangement during downstream processing. This makes the coverslip suited to microscopy-based comparisons between exposed and treated experimental conditions.
A typical workflow begins by growing adherent immune or host cells on the coverslip, followed by exposure to microbes or microbial products. Investigators then fix the cells, apply immunostaining when protein localization is being examined, and use light or fluorescence microscopy to record cellular or infection-related phenotypes. Each stage links culture conditions with visual readouts.
Microscopy on these substrates can provide several complementary readouts, including cellular morphology, protein localization, pathogen uptake, and inflammatory responses. These observations allow investigators to assess how exposure changes cells and whether a treatment alters the resulting phenotype. Because the readout is visual, the workflow connects cellular appearance or localization with the experimental condition.
In immunology and infection studies, the coverslip provides a platform for examining both host responses and microbial interactions. Researchers can culture immune or host cells, introduce microbes or microbial products, and compare resulting images across conditions. This supports analysis of infection phenotypes, immune activation, and treatment effects while preserving the cells’ microscopic context.