Transwell Epithelial Monolayer
Transwell Epithelial Monolayer - Related Videos
02:28
Assessing Transcytosis of E. coli Across Intestinal Epithelial Monolayers
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2025
Source: Islam, A., et al., Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates. J. Vis. Exp. (2023)This video demonstrates the procedure for assessing transcytosis of neonatal Escherichia coli across intestinal epithelial monolayers using transwell inserts, electrical resistance measurement, and bacterial quantification.
08:24
The Transwell Migration Assay
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2023
Cells migration in response to chemical cues is crucial to development, immunity and disease states such as cancer. To quantify cell migration, a simple assay was developed in 1961 by Dr. Stephen Boyden, which is now known as the transwell migration assay or Boyden chamber assay. This set-up consists an insert which separates the wells of a multiwell plate into top and bottom compartments. Cells whose migration is to be studied are seeded into the top compartment and the chemoattractant...
03:20
A Transwell Tissue Model for Studying Interactions Between Human Oral Biofilms and Epithelium
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2026
Source: Alshehri, M. et. al., Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro. J. Vis. Exp. (2025)This video demonstrates a co-culture model using multi-species oral biofilms and human oral epithelium. It highlights epithelial immune activation and enables the downstream analysis of host-pathogen interaction.
06:32
Development of Human Renal Tubular Epithelial Cell Primary Cultures in Monolayers and Three-Dimensional Conditions
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2025
Here, we describe the protocols for obtaining primary cultures of human renal tubular epithelial cells (HRTEC) from the kidney cortex to develop monolayers and three-dimensional cultures on an extracellular matrix (3D-HRTEC).
11:49
Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
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Cited by 5 •
2014
Human infection by Entamoeba histolytica leads to amoebiasis, a major cause of diarrhea in tropical countries. Infection is initiated by pathogen interactions with intestinal epithelial cells, provoking the opening of cell-cell contacts and consequently diarrhea, sometimes followed by liver infection. This article provides a model to assess the early host-pathogen interactions to improve our understanding of amoebiasis pathogenesis.
FAQs
Polarization gives the two sides different experimental meanings by maintaining distinct apical and basolateral compartments. Because epithelial cells form cell-cell junctions across the membrane, researchers can examine whether a substance, microbe, or immune signal moves directionally through the layer rather than simply contacting cells in an undifferentiated culture. This arrangement supports tissue-like transport analysis.
The membrane performs two functions at once: it supports epithelial cell growth and maintains separation between apical and basolateral compartments. Its pores create a controlled route for transport, so investigators can distinguish material that remains on one side from material that crosses the epithelial layer. This separation is central to studying translocation and barrier function.
Cell-cell junctions help the monolayer regulate passage between the two compartments. Their presence contributes to barrier integrity, while permeability measurements reveal how effectively the layer restricts or permits movement. Pairing permeability data with observations of cellular changes helps researchers connect barrier behavior with epithelial responses during experimental exposure.
To build the model, epithelial cells are grown on a porous membrane that separates apical and basolateral compartments. Once the cells form a monolayer with cell-cell junctions, researchers can introduce the substance, microbe, or immune signal of interest to one side and examine effects on the other. This workflow preserves directional sampling across the barrier.
This model is useful when the question concerns more than whether a microbe contacts epithelial cells. Investigators can assess pathogen attachment, determine whether organisms or their effects appear across the barrier, and examine epithelial responses in the presence of immune interactions. These readouts help relate infection behavior to mucosal defense and barrier function.
Permeability measurements describe barrier performance, while cytokine release reports signaling responses and cellular changes reveal effects on the epithelial layer. Considered together, these outcomes can distinguish altered transport from an inflammatory response or another cellular effect. The combined evidence supports interpretation of mucosal defense, infection mechanisms, and potential therapeutic strategies.