Yersinia Pestis Detection
Yersinia Pestis Detection - Related Videos
01:30
Improved Differential Isolation of Yersinia Species Using a Modified Cefsulodin-Irgasan-Novobiocin Agar
0 Views •
2025
Begin with cultures of Gram-negative Yersinia species and non-Yersinia Enterobacteriaceae.Using sterile needle inoculators, dot the cultures onto both traditional Cefsulodin-Irgasan-Novobiocin or CIN agar and modified CIN agar.Incubate the plates to promote bacterial growth.Both traditional and modified CIN agars support the growth of Yersinia and non-Yersinia Enterobacteriaceae that ferment mannitol to produce acidic byproducts.Acid production lowers the pH and triggers a color change,...
11:31
'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
0 Views •
Cited by 18 •
2011
A simple method for the identification of priority bacterial pathogens is to use genetically engineered reporter phage. These reporter phage, which are specific to their particular host species, are capable of rapidly transducing a bioluminescent signal response to host cells. Herein, we describe the use of reporter phage for the detection of Yersinia pestis.
12:23
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
0 Views •
Cited by 5 •
2015
Effector translocation into host cells via a type III secretion system is a common virulence strategy among gram-negative bacteria. A beta-lactamase effector fusion based assay for quantitative analysis of translocation was applied. In Yersinia infected cells, conversion of a FRET reporter by the beta-lactamase is monitored using laser scanning microscopy.
08:36
Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth
0 Views •
Cited by 2 •
2024
Bacteria colonize host tissues that vary in oxygen and iron bioavailability, yet most approaches to studying bacteria use aerated, rich media. This protocol describes culturing the human pathogen Yersinia pseudotuberculosis under varying iron concentrations and oxygen tension, and quantifying activity of the Yersinia type III secretion system, which is an important virulence factor.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
0 Views •
Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
FAQs
A positive culture provides evidence that viable Yersinia pestis is present in the tested material, rather than only showing bacterial DNA or an immune signal. This distinction matters for clinical, environmental, and animal samples because viability informs infection assessment, biosafety decisions, and public health action. Culture therefore supplies a different evidentiary layer from amplification and immunoassays.
PCR provides molecular evidence by amplifying species-specific Yersinia pestis DNA sequences. Unlike culture, this approach focuses on the organism’s genetic material rather than demonstrating viable bacteria. Its result is therefore most informative when interpreted alongside culture and immune-based findings, allowing investigators to compare molecular evidence with evidence of bacterial viability or host immune recognition.
Immunoassays can target either a bacterial component or the host response to infection. Detection of the F1 capsule protein represents identification of a Yersinia pestis antigen, whereas detection of host antibodies reflects immune recognition of the bacterium. Distinguishing these signals helps researchers assess active infection separately from evidence of prior exposure in immunology and infection studies.
Culture, PCR, and immunoassays answer different biological questions: culture demonstrates viable bacteria, PCR identifies species-specific DNA, and immunoassays detect bacterial antigen or host antibodies. Using these approaches together can provide a more informative interpretation than relying on a single signal. The combined evidence supports decisions involving diagnosis, surveillance, outbreak monitoring, and public health response.
The investigation should first identify whether the material is clinical, environmental, or animal in origin, because each context addresses a different surveillance or diagnostic question. Culture, PCR, and immunoassays can then be considered as complementary readouts for that sample. This approach links the selected evidence to infection assessment, environmental monitoring, or tracking in animal populations.
Detection results help connect individual findings with broader public health decisions. Identifying the bacterium in relevant samples can support timely treatment, contact tracing, biosafety decisions, and outbreak monitoring. When results from clinical, environmental, or animal sources are considered together, investigators can also characterize transmission and monitor this zoonotic pathogen across human and animal populations.
In this field, detection links the pathogen to the host response and to patterns of transmission. Bacterial culture and DNA amplification provide organism-focused evidence, while antigen and antibody assays add information about bacterial components or immune recognition. Comparing these signals helps distinguish active infection from prior exposure and supports research on transmission, surveillance, and outbreak characterization.