Zebrafish Inflammation Assay
Zebrafish Inflammation Assay - Related Videos
07:50
Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish
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Cited by 39 •
2012
Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.
09:38
An Assay for Lateral Line Regeneration in Adult Zebrafish
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Cited by 11 •
2014
Because many zebrafish models of neurological and non-neurological diseases are studied in the adult fish rather than the embryo/larvae, we developed a quantitative lateral line regenerative assay that can be applied to adult zebrafish disease models. The assay involved resolution at the 1) neuromast and 2) individual hair cell levels.
08:43
Boldness, Aggression, and Shoaling Assays for Zebrafish Behavioral Syndromes
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Cited by 18 •
2016
This manuscript describes the setup, implementation, and analysis of boldness, aggression, and shoaling in zebrafish and testing for the presence of a behavioral syndrome. A standardized approach for behavioral quantification will allow for easier comparison across studies. Modifications to this protocol are possible as each assay can be run individually.
01:38
Inflammation
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2019
Overview In response to tissue injury and infection, mast cells initiate inflammation. Mast cells release chemicals that increase the permeability of adjacent blood capillaries and attract additional immune cells to the wound or site of infection. Neutrophils are phagocytic leukocytes that exit the bloodstream and engulf invading microbes. Blood clotting platelets seal the wound and fibers create a scaffold for wound healing. Macrophages engulf aging neutrophils to end the acute inflammatory...
06:37
An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
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Cited by 11 •
2012
We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.
FAQs
The assay tracks measurable consequences of an induced inflammatory response, including immune-cell recruitment, tissue changes, and inflammatory signaling. Monitoring these features at multiple time points shows how the response develops rather than providing only a single endpoint. This temporal view helps investigators relate early cellular activity to later tissue-level or organism-level outcomes in zebrafish.
Immune-cell recruitment indicates how immune cells respond to the inflammatory stimulus, while tissue changes provide evidence of effects at the site or level of the organism. Examining both readout types gives a broader picture than relying on either alone. Their combined measurement can support studies of host responses and help identify mechanisms involved in inflammation.
A defined stimulus establishes the condition that initiates inflammation, allowing responses to be examined in relation to a known experimental trigger. The resulting changes can then be monitored and quantified across larvae or adults and over time. This organization helps researchers compare host responses systematically while investigating immune mechanisms in immunology and infection studies.
The workflow begins by exposing zebrafish larvae or adults to a defined inflammatory stimulus. Investigators then monitor selected features, such as immune-cell recruitment, tissue changes, or inflammatory signaling, over time and quantify the observed response. The assay therefore links experimental induction with longitudinal observation and measurement, while retaining whole-organism physiological context.
Researchers can apply the assay when they need to characterize host responses to pathogens or investigate immune mechanisms involved in infection. Its whole-organism format connects cellular immune activity with physiological outcomes, while accessible imaging supports observation of responses in the animal. These features make it useful for examining infection-related inflammation rather than isolated immune events alone.
The assay can be used to evaluate anti-inflammatory or anti-infective compounds by measuring how treatment-associated responses relate to induced inflammation. Readouts such as immune-cell recruitment, tissue changes, or inflammatory signaling provide observable outcomes for comparison. Because the design is scalable and supports whole-organism observation, it can connect compound effects with physiological responses in zebrafish.