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JoVE Journal
Neuroscience

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An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
 

An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium

Article doi: 10.3791/4242
October 24th, 2012

Summary October 24th, 2012

We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.

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