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An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
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Neuroscience
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JoVE Journal Neuroscience
An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium

An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium

DOI:
10.3791/4242-v

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06:37 min

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October 24, 2012

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,
1Department of Biology,Massachusetts Institute of Technology, 2Whitehead Institute of Biomedical Research

Chapters

  • 00:05Title
  • 01:05Preparing Injection Needles and Embryos
  • 02:22Injecting Brain Ventricles
  • 03:22Image and Quantification of Dye Movement
  • 04:44Representative Permeability Results of Neuroepithelium
  • 06:18Conclusion

Summary

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Automatic Translation

We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.

Tags

PermeabilityZebrafish Embryonic NeuroepitheliumBrain Ventricular SystemBrain VentriclesCerebrospinal Fluid (CSF)Protein-rich FluidNeural Tube FormationClaudin 5aNaK-ATPaseTight Junction FormationVentricular Dye Retention AssayMyosin Inhibition

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