Anterior Chamber Cannulation
Anterior Chamber Cannulation - Related Videos
05:27
In Vivo Imaging of Liver Spheroids Engrafted in the Anterior Chamber of the Mouse Eye
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2024
Here, we describe a platform that allows noninvasive in vivo imaging of liver spheroids engrafted in the anterior chamber of the mouse eye. The workflow spans from generating spheroids from primary liver cells to transplantation into the mouse eye and in vivo imaging at cellular resolution by confocal microscopy.
09:49
Isolation and Cannulation of Cerebral Parenchymal Arterioles
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Cited by 28 •
2016
This manuscript describes a simple and reproducible protocol for isolation of intracerebral arterioles (a group of blood vessels encompassing parenchymal arterioles, penetrating arterioles and pre-capillary arterioles) from mice, to be used in pressure myography, immunofluorescence, biochemistry, and molecular studies.
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
02:05
Intraduodenal Cannulation in a Rat Model for Immunopathology Studies
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2025
This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.
05:18
Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
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Cited by 17 •
2011
A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.
FAQs
Pressure control depends on managing the movement of fluid through the cannula while maintaining access to the anterior chamber. This allows the surgeon to regulate pressure rather than leave it entirely to uncontrolled fluid movement during the procedure. The approach is therefore useful when stable pressure supports surgical control or pressure-management work.
Positioning determines whether the cannula remains in the intended fluid-filled space without contacting nearby tissues. Inaccurate placement or excessive manipulation can injure the corneal endothelium, iris, lens, or other intraocular structures. Careful control is consequently central to preserving ocular tissues while achieving fluid access, exchange, or material handling.
Controlled exchange allows aqueous humor to be managed under direct surgical control rather than through an uncontrolled opening. Depending on the purpose of the procedure, this can support pressure regulation or permit the removal and delivery of material. The same principle also makes the technique useful for investigating ocular fluid physiology.
The procedure requires selecting an appropriate surgical entry, introducing a fine cannula into the anterior chamber, and manipulating it with precision. The operator must maintain direct control of the cannula and any fluid movement while avoiding contact with vulnerable intraocular structures. These considerations support safe access during anterior segment procedures and experimental work.
In ophthalmic medicine, the technique may support anterior segment surgery and procedures concerned with intraocular pressure. Its ability to provide controlled access to aqueous humor also permits fluid exchange or the delivery and removal of material. The specific use depends on whether the immediate objective is surgical assistance, pressure management, or controlled intraocular manipulation.
Experimental investigators can use the cannula to obtain controlled access to aqueous humor and regulate fluid movement within the anterior chamber. This provides a way to examine ocular fluid behavior under direct surgical control. In that context, the technique connects microsurgical access with studies of pressure regulation and anterior-segment physiology.