Blood Volume Quantification
Blood Volume Quantification - Related Videos
08:38
Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
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Cited by 28 •
2015
Here we describe a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay to quantify the immunosuppressant tacrolimus in dried blood spots using a simple manual protein precipitation step and online column extraction.
01:21
Measuring Cerebral Blood Volume in the Auditory Cortex Using Vascular Space Occupancy
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2025
Position a human participant’s head within the head coil of a functional magnetic resonance imaging, or fMRI, system.Employ vascular space occupancy or VASO, a technique to measure changes in cerebral blood volume, or CBV, the blood volume within the brain.Begin by applying an inversion recovery pulse to suppress signals from blood, allowing only signals from the surrounding tissue within the region to be detected.Next, use MRI-compatible earbuds to deliver auditory stimuli.The stimuli reach...
08:54
Measuring Blood Pressure in Mice using Volume Pressure Recording, a Tail-cuff Method
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Cited by 175 •
2009
The CODA 8-Channel High Throughput Non-Invasive Blood Pressure system measures the blood pressure in up to 8 mice or rats simultaneously. This tail-cuff system uses Volume Pressure Recording (VPR) to measure the blood pressure by determining the tail blood volume.
12:45
Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images
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Cited by 10 •
2022
Tunneling nanotubes (TNTs) are primarily open-ended F-actin membrane nanotubes that connect neighboring cells, facilitating intercellular communication. The notable characteristic that distinguishes TNTs from other cell protrusions is the hovering nature of the nanotubes between cells. Here, we characterize TNTs by constructing a 3D volume view of confocal z-stack images.
04:09
Large Volume Blood Collection from Swine for In Vitro Applications: Use of Intracardiac Cannulation and a Vacuum Pump as a Terminal Procedure
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2025
Here we present a method to collect a large volume of blood from swine using percutaneous intracardiac cannulation connected to a vacuum pump, as a terminal procedure.
FAQs
The calculation pairs a known quantity of tracer with its concentration after the tracer has circulated and mixed. Measuring the concentration at a defined time provides the basis for estimating the volume in which that tracer is distributed. Depending on the tracer and measurement target, the result can represent total blood volume, plasma volume, or red cell volume.
Separating these components shows whether a change reflects the liquid portion of blood, the cellular portion, or both. That distinction can add clinical context when evaluating anemia, polycythemia, dehydration, or fluid overload. A total-volume estimate alone may not identify which compartment contributes most to an abnormal circulating blood volume.
Body weight and vital signs can provide indirect estimates, but they may not adequately represent an individual’s circulating volume. Direct measurement or estimation through an indicator-dilution approach supplies quantitative information about blood, plasma, or red cell volume. This added information is particularly relevant when hemorrhage or major fluid changes make clinical interpretation more difficult.
The process begins by introducing a known quantity of tracer into the circulation. The tracer is then allowed to mix, and a measurement is obtained at a defined time. The resulting concentration is used for the volume calculation. Depending on the measurement objective, the analysis yields an estimate of total blood volume, plasma volume, or red cell volume.
Clinical uses include assessing hemorrhage, anemia, polycythemia, dehydration, and fluid overload. The measurements can also characterize circulatory changes that are not adequately captured by routine indirect estimates. By supplying quantitative volume information, the assessment can support diagnosis and help clinicians interpret whether a patient’s circulating blood components are substantially altered.
Quantified blood, plasma, or red cell volume can provide additional evidence when clinicians evaluate blood loss or abnormal circulating volume. This information may support transfusion decisions rather than relying only on body weight or vital signs. Repeated or appropriately timed measurements can also improve monitoring of circulatory changes during medical care, when such assessment is clinically indicated.