Ex Vivo Tissue Culture
Ex Vivo Tissue Culture - Related Videos
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Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery
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Cited by 4 •
2010
This article describes the culture of patient tissue slices for gene delivery studies and subsequent analysis of gene expression using IVIS bioluminescence imaging.
08:28
Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
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Cited by 28 •
2016
The functional behavior of cells in culture can be improved by culturing in more in vivo-like 3-dimensional culture environments16-21. This manuscript describes the set-up and operation of a hollow fiber bioreactor system for in vivo-like mammalian tissue culture.
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Histotypic Tissue Culture
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2023
Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.
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Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
07:45
Explant Culture of Neural Tissue
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2023
The intricate structure of the vertebrate nervous system arises from a complex series of events involving cell differentiation, cell migration, and changes in cell morphology. Studying these processes is essential to our understanding of nervous system function as well as our ability to diagnose and treat disorders that result from abnormal development. However, neural tissues are relatively inaccessible for experimental manipulations, especially in embryonic mammals. As a result, many...
FAQs
Native cell interactions allow tissue behavior to be examined in a more organized biological setting than isolated cells provide. Communication among neighboring cells and the retained tissue structure can influence how disease-related changes, treatments, or toxic exposures appear. This makes the model useful when researchers need observations that better reflect tissue-specific responses rather than responses from individual cells alone.
These conditions directly influence whether the tissue remains viable and continues its tissue-specific functions. If temperature, pH, oxygen, or nutrient availability is not appropriately regulated, cellular activity and tissue responses may change, making results difficult to interpret. Controlled conditions therefore support consistent observations when comparing disease states, drug effects, or toxic responses across experiments.
The main distinction is the level of biological organization retained. Isolated cell cultures focus on individual cells, whereas ex vivo tissue samples preserve native cellular relationships and tissue structure to a greater extent. Consequently, ex vivo studies can reveal coordinated tissue responses while still allowing researchers to observe and control experimental conditions directly outside the organism.
A basic workflow includes collecting the tissue, placing it in a suitable culture medium, and maintaining it under regulated temperature, pH, oxygen, and nutrient conditions. Researchers then observe whether the sample remains viable and continues tissue-specific activity while applying the experimental conditions of interest. This controlled sequence supports direct assessment of tissue responses outside the body.
Ex vivo cultures can provide information about disease mechanisms, tissue responses, drug effects, and toxicity. Because the sample remains organized as tissue, observations may connect an experimental exposure with changes in tissue-level behavior rather than only isolated cellular effects. The approach also permits direct comparison of responses under controlled laboratory conditions, supporting evaluation of therapeutic strategies.
Medical researchers can apply this approach to tissue engineering, personalized medicine, and the evaluation of therapeutic strategies. It is especially relevant when retaining native tissue organization may improve understanding of how a treatment or harmful exposure affects the sample. Direct experimental control also helps investigators examine responses before considering how findings may inform broader medical research.