Fluoroscopic Guidance

Fluoroscopic guidance is a medical imaging technique that uses a continuous or pulsed X-ray beam to produce real-time moving images of internal structures, helping clinicians perform procedures with precision. During an intervention, the fluoroscope projects X-rays through the body, while a detector converts transmitted radiation into a live image; radiopaque contrast agents may be introduced to outline blood vessels, ducts, or other target anatomy. Physicians use this guidance for catheter placement, angiography, injections, biopsies, and orthopedic procedures. By visualizing instruments and anatomy as they move, fluoroscopy supports accurate treatment, although radiation exposure requires dose optimization and protective practices.

Fluoroscopic Guidance - Related Videos

Research

JoVE Journal - Medicine
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Non-fluoroscopic Catheter Tracking for Fluoroscopy Reduction in Interventional Electrophysiology

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Cited by 4 •

2015

Radiation exposure is an underestimated risk in complex ablation procedures. Here, we describe a protocol to significantly decrease fluoroscopy time and dosage for both the patient and the lab staff by using a novel non-fluoroscopic catheter visualization system.

Research

JoVE Journal - Medicine
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X-ray Dose Reduction through Adaptive Exposure in Fluoroscopic Imaging

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Cited by 2 •

2011

We are developing a dynamic adaptive exposure technique using our scanning beam digital X-ray system. Rather than exposing an object uniformly, the exposure is adapted depending on the opacity of the object. Here we show an experiment on an anthropomorphic phantom that resulted in a dose saving of 30%.

Research

JoVE Journal - Bioengineering

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

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Cited by 9 •

2016

This protocol describes a bioprinting methodology using an automated robotic depositing system that incorporates etched topographical guidance cues with the precision deposition of a cell bearing hydrogel bioink. The printed cells are directly delivered to the etched features and are able to sense and orientate with them.

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

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Cited by 10 •

2014

We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

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Cited by 10 •

2019

Sperm must successfully navigate through the oviduct to fertilize an oocyte. Here, we describe an assay for measuring sperm migration within the C. elegans hermaphrodite uterus. This assay can provide quantitative data on sperm distribution within the uterus after mating, as well as on speed, directional velocity, and reversal frequency.

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