Graft Perfusion
Graft Perfusion - Related Videos
08:12
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
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Cited by 43 •
2015
The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
09:29
Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts
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Cited by 31 •
2014
Marginal grafts, such as fatty livers, grafts from older donors, or livers retrieved after cardiac death (DCD) tolerate conventional, cold static storage only poorly. We developed a novel model of subnormothermic ex vivo liver perfusion for preservation, assessment, and repair of marginal liver grafts prior to transplantation.
06:22
Modified Langendorff Perfusion for Extended Perfusion Times of Rodent Cardiac Grafts
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Cited by 2 •
2024
This article demonstrates the feasibility of achieving longer perfusion times (4 h) of murine cardiac grafts without function loss by employing lower (30-35 mmHg) than physiological (60-80 mmHg) perfusion pressures during Langendorff.
07:32
Porcine Normothermic Isolated Liver Perfusion
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Cited by 3 •
2023
The porcine model of liver normothermic machine perfusion (NMP), described here, can be successfully used to study NMP as a preservation strategy, a tool for viability assessment, and a platform for organ repair. It holds a high translational value, however it is technically challenging and labor-intensive.
12:13
Ex Situ Normothermic Machine Perfusion of Donor Livers
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Cited by 21 •
2015
Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
FAQs
Controlled pressure and flow determine how effectively preservation fluid moves through the graft’s vascular network. Monitoring these variables can reveal changes in vascular resistance, which may indicate how readily the tissue accepts perfusion. Maintaining controlled conditions helps generate interpretable information about graft condition rather than exposing the organ to uncontrolled mechanical stress.
Oxygenated fluid can support cellular metabolism during preservation, while chilled fluid reduces the conditions that promote metabolic activity. The selected perfusate and temperature therefore influence the physiological state being maintained and the measurements obtained. These variables help investigators evaluate tissue condition while preserving the graft before implantation.
Metabolism and vascular resistance provide functional information that static inspection or storage alone may not show. Changes in these parameters can help characterize tissue condition during preservation and contribute to viability assessment. Together with the graft’s overall condition, such measurements can support decisions about whether an organ is suitable for transplantation.
Conventional cold storage primarily preserves the graft under chilled conditions, whereas machine perfusion adds active circulation through the tissue. This circulation creates an opportunity to monitor metabolism, vascular resistance, and other indicators of condition. As a result, perfusion can be especially valuable when cold storage alone may provide insufficient information or preservation support.
A typical workflow connects the graft to a pump, circulates an oxygenated or chilled preservation solution through the tissue, and maintains controlled pressure and flow. During perfusion, investigators assess metabolic activity, vascular resistance, and graft condition. These observations can then inform preservation decisions and help determine whether the tissue should proceed toward transplantation.
This approach is particularly useful for assessing marginal organs, meaning grafts whose condition may make their suitability less certain. Active perfusion supplies measurable information that can support selection decisions and guide whether preservation should continue. It therefore expands evaluation beyond a simple storage interval when conventional cold preservation may be insufficient.
Beyond preservation and assessment, the perfusion setup can provide a controlled platform for delivering treatments to the graft before implantation. Because fluid is circulated through the transplanted tissue, investigators can study treatment-related effects alongside metabolic activity, vascular resistance, and overall condition. This creates a bridge between preservation procedures and pretransplant therapeutic investigation.