Hindlimb Amputation
Hindlimb Amputation - Related Videos
06:49
Rat Hindlimb Below-Knee Amputation Model and Immediate Targeted Muscle Reinnervation
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2026
This protocol demonstrates how to perform a below-knee amputation in rats and to immediately perform targeted muscle reinnervation (iTMR) to investigate strategies to treat and prevent amputation-related pain and to better understand pain mechanisms associated with amputation.
07:57
Methods for Acute and Subacute Murine Hindlimb Ischemia
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Cited by 31 •
2016
Surgical induction of hindlimb ischemia in the mouse is useful to examine angiogenesis, however this is compromised in certain inbred mouse strains that display marked ischemia-induced tissue necrosis. Methods are described to induce subacute limb ischemia using ameroid constrictors to circumvent this problem through the induction of gradual arterial occlusion.
06:53
Heterotopic Hindlimb-to-Neck Transplantation in the Mouse: A Supermicrosurgical Model of Vascularized Composite Allotransplantation
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2026
We present a reproducible mouse model of heterotopic hindlimb-to-neck vascularized composite allotransplantation. This supermicrosurgical protocol, involving 0.3-0.5 mm vessel anastomoses, integrates nylon filament guidance and venous cuffing. It provides a reliable platform for translational studies on tolerance, ischemia-reperfusion injury, and immunological mechanisms in vascularized composite allotransplantation.
12:51
Murine Model of Hindlimb Ischemia
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Cited by 201 •
2009
The surgical procedure for induction of unilateral hindlimb ischemia is demonstrated, with confirmation of ischemia by laser Doppler perfusion imaging.
03:40
Hindlimb Skeletal Muscle Extraction: A Procedure to Excise Hindlimb Skeletal Muscles from Murine Model
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2023
In this video, we describe the procedure to excise hindlimb skeletal muscles from a mouse model. The excised muscles can be stored at lower temperatures until further downstream...
FAQs
Shaping the remaining bone and muscle helps create a stable residual limb, which is important for wound closure, healing, and later rehabilitation. The operation therefore does more than remove visibly damaged tissue: clinicians also prepare the remaining structures and close the wound in a way that supports recovery. This foundation can help patients work toward mobility and independence.
Bleeding control is a distinct intraoperative priority because tissue removal must be followed by preparation and closure of the remaining limb. By incorporating bleeding control with bone and muscle shaping, clinicians create conditions for the wound to close and heal. This coordinated approach supports a stable residual limb for rehabilitation rather than treating removal as the only surgical objective.
The amount removed depends on where damaged, infected, nonviable, or poorly perfused tissue prevents safe limb preservation. Removing only the affected portion may leave a stable residual limb, whereas more extensive disease or injury may require removal of the entire lower limb. This decision links surgical extent with safety and subsequent rehabilitation.
Clinicians excise damaged tissue, control bleeding, shape the remaining bone and muscle, and close the wound. The sequence moves from removing tissue that cannot safely remain to preparing the residual limb for healing. Proper closure then supports healing at the surgical site while the patient enters wound care and rehabilitation.
After surgery, wound management helps clinicians follow healing, while physical therapy addresses recovery of movement and function. The care plan also includes assessment for prosthetic fitting when appropriate. Together, these measures connect wound recovery with rehabilitation, helping patients work toward restored mobility, greater independence, and improved quality of life.
Clinicians can judge the broader result by considering whether the procedure relieved pain, removed nonviable or infected tissue, and prevented further complications. Functional recovery adds another dimension: physical therapy and possible prosthetic fitting are directed toward mobility and independence. These outcomes explain why follow-up extends beyond incision healing to the patient’s quality of life.