Immunodeficient Mice
Immunodeficient Mice - Related Videos
06:55
Bioengineering Human Microvascular Networks in Immunodeficient Mice
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Cited by 21 •
2011
Here, we describe a methodology to deliver human cord blood-derived endothelial colony-forming cells (ECFCs) and bone marrow-derived mesenchymal stem cells (MSCs), embedded in a collagen/fibronectin gel, subcutaneously into immunodeficient mice. This cell/gel combination generates a human vascular network that connects with the mouse vasculature.
08:02
Th17 Inflammation Model of Oropharyngeal Candidiasis in Immunodeficient Mice
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Cited by 11 •
2015
Although Candida infection models are available to study host immune resistance, a model to study T cell mediated immunopathology in the context of Candida infection is absent. Here we describe a method to establish Th17 immunopathology associated with oral Candida infection in immunodeficient mice.
10:35
Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice
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Cited by 15 •
2013
Human tumor xenografts in immunodeficient mice are valuable tools to study cancer biology. Specific protocols to generate subcutaneous and intrahepatic xenografts from human hepatocellular carcinoma cells or tumor fragments are described. Liver regeneration induced by partial hepatectomy in recipient mice is presented as a strategy to facilitate intrahepatic engraftment.
08:37
Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
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Cited by 17 •
2015
Here, we present a protocol to induce colonic inflammation in mice by adoptive transfer of syngeneic CD4+CD45RBhigh T cells into T and B cell deficient recipients. Clinical and histopathological features mimic human inflammatory bowel diseases. This method allows the study of the initiation of colonic inflammation and progression of disease.
07:48
Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice
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Cited by 4 •
2019
Complex human diseases can be challenging to model in traditional laboratory model systems. Here, we describe a surgical approach to model human muscle disease through the transplantation of human skeletal muscle biopsies into immunodeficient mice.
FAQs
Reduced activity in lymphocytes and other immune components limits the rejection of transplanted human material. This creates an experimental setting in which human tumors, organoids, or patient-derived cells can become established and remain available for observation. Researchers can then examine disease behavior or therapeutic responses in a living system rather than relying only on isolated cells or tissues.
The condition may arise through inherited genetic mutations, targeted treatments, or defects affecting lymphocytes and other immune components. These different causes alter immune activity in distinct ways, so the resulting model determines how effectively transplanted human material can be maintained. Selecting among such models allows researchers to match the degree or type of immune impairment to the study objective.
Controlled conditions help researchers relate observed disease growth or treatment responses to the experimental intervention while limiting variation from uncontrolled immune activity. Because the animals provide a consistent setting for human tumors, organoids, or patient-derived cells, investigators can compare outcomes across experimental groups and examine disease mechanisms under conditions designed for the specific research question.
A study generally begins by establishing human material, such as a tumor, organoid, or patient-derived cell population, in an appropriate mouse model. Researchers then monitor the resulting disease-related behavior and assess responses to a candidate treatment or other intervention. The findings can provide preclinical evidence about disease mechanisms and therapeutic activity before clinical testing.
Patient-derived cells and organoids allow investigators to study cancer material that reflects a particular patient or disease context. When established in immunodeficient mice, these materials can support analysis of tumor growth and treatment response in a living experimental system. This helps connect laboratory observations with questions about disease behavior and potential therapeutic strategies.
Their applications include infectious disease, transplantation, and immune-based therapies, in addition to cancer research. Investigators can use the models to examine how human cells or tissues behave in a host with reduced immune activity, explore disease mechanisms, and evaluate therapeutic responses. This broad use makes them relevant to several stages of medical investigation before clinical testing.