Lumbar Ganglia
Lumbar Ganglia - Related Videos
04:01
Isolation of Cells from Sensory Ganglia
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2025
This video demonstrates the isolation of cells from the sensory ganglia of mice. Ganglia are subjected to enzymatic digestion to dissolve the extracellular matrix. Single cells are then collected via filtering through a cell strainer followed by density gradient centrifugation for debris removal. Finally, washing and centrifugation are done to collect the isolated cells for future analysis.
05:18
Establishing a Primary Dorsal Root Ganglia Cell Culture from a Rat Spinal Column
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2025
This video demonstrates a technique for isolating and culturing dorsal root ganglia (DRG) cells from the rat spinal column. DRGs excised from the spinal column are treated with enzymes and mechanically dissociated to obtain a single-cell suspension. The cells are cultured in a medium containing inhibitors and growth factors that limit glial cell proliferation and promote neuronal growth, respectively.
12:00
Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
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Cited by 8 •
2016
This manuscript describes how to prepare intact dorsal root ganglia for patch clamp recordings. This preparation maintains the microenvironment for neurons and satellite glial cells, thus avoiding the phenotypic and functional changes seen using dissociated DRG neurons.
03:02
Intrathecal Placement of Lumbar Drain: A Technique to Withdraw Cerebrospinal Fluid from the Lumbar Spine in Porcine Model
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2025
This video describes the technique of intrathecal placement of a lumbar drain in a porcine ischemic model, which helps in draining the cerebrospinal fluid to a drainage system. The cerebrospinal fluid pressure is monitored by the drainage system, and fluid is drained to maintain a constant cerebrospinal fluid pressure.
08:10
Hydraulic Extrusion of the Spinal Cord and Isolation of Dorsal Root Ganglia in Rodents
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Cited by 110 •
2017
Here, we present a protocol for hydraulic extrusion of the spinal cord as well as identification and isolation of specific dorsal root ganglia (DRGs) in the same rodent. Compared to standard spinal cord isolation methods, this method is significantly faster and reduces the risk of tissue damage.
FAQs
Preganglionic sympathetic fibers reach the ganglion and synapse with nerve-cell bodies there. The resulting postganglionic neurons carry the signal onward through connected nerves rather than ending at the ganglion. This relay creates a functional transition point, allowing sympathetic activity to influence lower-body targets while preserving the organization of the autonomic pathway.
Their downstream sympathetic activity can alter blood-vessel tone, promote sweating, and affect visceral activity in the lower body. These effects matter because the same neural region can influence several involuntary functions, not just one organ. Consequently, findings involving circulation, sweating, or visceral regulation may be considered in relation to autonomic function.
Their location makes relationships with nearby nerves and vessels clinically important. During spinal, retroperitoneal, or vascular procedures, an approach that reaches the lumbar region may affect these neighboring structures as well as the ganglia. Anatomical knowledge therefore helps clinicians anticipate possible autonomic or vascular consequences when planning or interpreting such procedures.
A lumbar sympathetic block can serve either an evaluative or therapeutic purpose. Clinicians may use it to assess whether sympathetic pathways contribute to selected lower-limb pain or circulation disorders, or to treat those disorders. Its value therefore lies not only in symptom management but also in linking clinical findings with sympathetic involvement.
It may be considered when lower-limb pain or a circulation disorder requires assessment of sympathetic involvement. In that setting, the block provides a focused clinical test and may also offer treatment. This makes it relevant when clinicians need information about sympathetic contribution alongside an attempt to address the patient’s condition.
Because they participate in sympathetic control of lower-body vascular, sweating, and visceral responses, lumbar ganglia provide an anatomical reference when autonomic function appears abnormal. Their role helps clinicians connect those involuntary findings with a specific portion of the sympathetic trunk. This context can support clinical evaluation without treating the ganglia as an isolated system.