Metabolic Analyzer
Metabolic Analyzer - Related Videos
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
04:41
Real-Time Monitoring of Energy Metabolism in Human NK Cells Using an Extracellular Flux Analyzer
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2025
This video demonstrates the method to analyze energy metabolism in activated human NK cells using an extracellular flux analyzer. Different inhibitors of the electron transport chain complexes impact ATP synthesis, which is measured through the oxygen consumption rate or OCR, providing insights into cellular metabolism and bioenergetics.
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
09:43
Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
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Cited by 5 •
2022
Real-time cell metabolic flux assay measures the oxygen consumption rate and extracellular acidification rate, which corresponds to mitochondrial and glycolytic adenosine triphosphate production, using pH and oxygen sensors. The manuscript explains a method to understand the energy status of osteoblasts and the characterization and interpretation of the cellular bioenergetic status.
FAQs
Each measured variable acts as a different readout of metabolic activity. Oxygen consumption and carbon dioxide production provide gas-based evidence related to respiration, whereas extracellular acidification, glucose, and lactate indicate changes in the chemical environment or substrate use. Considering these readouts together can reveal shifts in energy pathways rather than relying on one signal alone.
Defined conditions make measurements interpretable because the observed metabolic change can be considered against a controlled experimental setting. This is especially important when comparing cells, tissues, or biological samples, or when examining responses to a therapeutic compound. Consistent conditions help researchers attribute differences to the biological question being tested.
Platform choice determines which indicators are available and how they are detected. Electrochemical, optical, or enzymatic sensors may be used to quantify oxygen, carbon dioxide, extracellular acidification, glucose, or lactate. This matters when planning an experiment because the selected readout must match whether the study focuses on respiration, energy use, or a metabolic response.
Researchers should identify whether the experiment concerns cells, tissues, or another biological sample, then select the metabolic indicators relevant to the question. They also need a defined experimental condition for interpreting changes. This planning links the instrument's measurements to a specific pathway, disease mechanism, biomarker question, or treatment response.
These measurements are suited to studies of mitochondrial function and cellular respiration, as well as metabolic disease and cancer biology. They can also be used to examine how biological samples respond to therapeutic compounds. In each case, the value lies in quantifying altered energy-related activity to investigate mechanisms or compare biological responses.
Quantitative metabolic changes can provide evidence that a disease process alters energy pathways or that a therapeutic compound changes cellular behavior. Researchers may use those patterns to investigate candidate biomarkers and evaluate treatment effects. The measurements support comparisons between defined conditions and help connect metabolic findings with broader medical research goals.