Metabolic Biomarkers
Metabolic Biomarkers - Related Videos
08:29
Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD
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Cited by 29 •
2012
We describe a rat model of post traumatic stress disorder (PTSD) that reveals the persistent alterations in neuroendocrine function and the delayed long-term, exaggerated fear response, characteristic of PTSD patients. The animal model and methods described here are useful for correlating biomarkers in brain nuclei, which are mechanistic but cannot be measured in patients, with biomarkers in peripheral white blood cells, which can.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
00:52
What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
Different biomarker classes provide complementary metabolic information. Metabolites and nutrient-related compounds can reflect biochemical resources and products, whereas hormones and enzymes can indicate regulatory activity or cellular processes. Measuring these signals in blood, urine, tissue, or other biological samples allows investigators to examine metabolism across physiological and disease-related states.
Changes in concentration can reveal altered biochemical pathways, energy balance, inflammation, or organ function. Interpreting a pattern of metabolic signals may therefore provide broader information than examining a single molecule in isolation. In medicine, these shifts can help distinguish physiological states from disease-related changes and support assessment of pathology.
Mass spectrometry, nuclear magnetic resonance, and immunoassays provide analytical routes for identifying and quantifying metabolic signals. Their inclusion in biomarker studies supports measurement of diverse molecules and biochemical patterns across biological samples. The resulting data can be used to characterize metabolic changes and develop more precise diagnostic tools.
A basic investigation involves selecting an appropriate biological sample, such as blood, urine, or tissue, then applying an analytical method to identify and quantify relevant signals. Researchers interpret the measured concentrations or biochemical patterns in relation to physiological or disease-related states. This workflow supports detection, risk assessment, and evaluation of organ or cellular changes.
Metabolic biomarkers can support several medical decisions: detecting disease, estimating risk, selecting treatments, and monitoring therapeutic response. Their value comes from connecting measurable biochemical changes with health or pathology. Because the signals may reflect altered pathways, energy balance, inflammation, or organ function, they can contribute information at multiple stages of patient assessment.
These measurements can help connect an individual's biochemical profile with disease-related changes or treatment response. That information supports treatment selection and may improve monitoring during therapy. At a broader research level, identifying and quantifying consistent metabolic patterns contributes to the development of more precise diagnostic tools and advances personalized medicine.