Normal Omentum Fibroblasts
Normal Omentum Fibroblasts - Related Videos
11:31
Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting (FACS)
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Cited by 79 •
2013
Cancer Associated Fibroblasts (CAFs) facilitate tumor initiation, growth and progression through signaling that promotes proliferation, angiogenesis, and inflammation. Here we describe a method to isolate pure populations of normal fibroblasts and CAFs from fresh mouse and human tissues by cell sorting, using PDGFRα as a surface marker.
03:18
Modeling Ovarian Cancer Cell Colonization of Omentum: An Ex Vivo Method to Establish Milky Spot Colonization of Fluorescently Labeled Ovarian Cancer Cells in Murine Omentum Explants
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2023
In this video, we establish an ex vivo omental organ culture overlaid with GFP-expressing ovarian cancer cells to study ovarian cancer colonization within the omental explant. This protocol analyzes immune cell structures within the omentum known as milky spots that promote metastases.
04:36
Mesothelial Cell Isolation: A Technique to Isolate Primary Mesothelial Cells From Human Omentum Tissue Specimen
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2023
This video describes the protocol to isolate primary human mesothelial cells from the human omentum specimen. The mesothelial cells can be used to establish an in vitro ovarian cancer model to investigate ovarian cancer cell adhesion, invasion, and proliferation.
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
06:25
A Method for Islet Transplantation to the Omentum in Mouse
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Cited by 4 •
2019
A method for the omental transplantation of islets in a mouse is introduced. The isolated islets are mixed with hydrogel and the mixture is placed into the omental pouch of a diabetic mouse. Then, the blood glucose is monitored, and immuno-histochemical analysis is performed.
FAQs
These cells integrate signals from their physical environment with cues from inflammatory cytokines. Mechanical inputs can influence tissue maintenance and repair-related behavior, while cytokine responses help regulate inflammation and scarring. Studying both signal types is important because fibroblasts operate within changing abdominal tissue conditions rather than responding to a single stimulus.
Collagen production and remodeling give omental fibroblasts a way to influence extracellular-matrix organization. That matrix helps maintain tissue structure, but altered remodeling can also contribute to fibrosis, a process involving excessive scar-like tissue formation. Measuring matrix changes therefore connects cellular activity with larger changes in abdominal tissue architecture.
Mechanical responsiveness makes normal omentum fibroblasts relevant to questions about how tissue conditions shape repair. Their behavior can be examined alongside inflammatory signaling to distinguish effects linked to physical strain from those associated with cytokine exposure. This combined perspective helps explain why the same stromal cell population may participate in maintenance, wound repair, or fibrotic remodeling under different conditions.
Researchers can culture normal omentum fibroblasts as a healthy reference model, then analyze their cellular behavior alongside diseased counterparts. This comparison establishes a baseline for identifying changes associated with disease, including altered stromal contributions to inflammation, scar formation, or fibrosis. It can also support evaluation of how candidate therapies affect abnormal tissue-related behavior.
In tumor microenvironments, fibroblast activity can be examined as part of the surrounding stromal context. Normal omentum fibroblasts provide a nonmalignant comparator for asking how stromal behavior differs when associated with tumor-related conditions. This comparison may clarify whether changes involve matrix remodeling, inflammatory signaling, or other tissue-support functions described in the model.
Therapy-related studies can use normal omentum fibroblasts to establish how a nonmalignant stromal reference responds before interpreting disease-associated effects. Researchers can compare cellular behavior under healthy and diseased contexts and examine consequences for inflammation, wound repair, scar formation, or fibrosis. Such comparisons help determine whether a potential treatment changes stromal processes linked to tissue maintenance or pathological remodeling.