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Chapters
- 00:05Title
- 01:45Dissecting Mammary and Skin Tissue from Mice
- 03:34Preparation of Mammary Gland/Skin Single Cell Suspension
- 04:49Staining Cells
- 07:07Isolation of Cells by FACS
- 08:47Results: Isolation of Highly Enriched Populations of Tissue Fibroblasts by FACS
- 10:49Conclusion
Cancer Associated Fibroblasts (CAFs) facilitate tumor initiation, growth and progression through signaling that promotes proliferation, angiogenesis, and inflammation. Here we describe a method to isolate pure populations of normal fibroblasts and CAFs from fresh mouse and human tissues by cell sorting, using PDGFRα as a surface marker.
Tags
IsolationNormal FibroblastsCancer-associated FibroblastsFluorescence Activated Cell SortingFACSTumor StromaBreast CarcinomaPoor PrognosisMyofibroblast Markerα-SMABone Marrow-derived CellsTumor MicroenvironmentTumor InitiationTumor GrowthTumor-promoting InflammationCAF-specific MarkersHeterogeneityGene Expression Profiling
