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Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting (FACS)
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Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting (FACS)

Article DOI: 10.3791/4425-v • 11:31 min • January 14th, 2013
January 14th, 2013 •
1, 1, 1, 1, 1
1Department of Pathology, Sackler School of Medicine, Tel Aviv University

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Summary
Automatic Translation

Cancer Associated Fibroblasts (CAFs) facilitate tumor initiation, growth and progression through signaling that promotes proliferation, angiogenesis, and inflammation. Here we describe a method to isolate pure populations of normal fibroblasts and CAFs from fresh mouse and human tissues by cell sorting, using PDGFRα as a surface marker.

Tags

Isolation Normal Fibroblasts Cancer-associated Fibroblasts Fluorescence Activated Cell Sorting FACS Tumor Stroma Breast Carcinoma Poor Prognosis Myofibroblast Marker α-SMA Local Tissue Fibroblasts Bone Marrow-derived Cells Tumor Microenvironment Tumor Initiation Tumor Growth Tumor-promoting Inflammation CAF-specific Markers Heterogeneity Gene Expression Profiling
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