Organ Bath Myography
Organ Bath Myography - Related Videos
07:25
Assessing Murine Resistance Artery Function Using Pressure Myography
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Cited by 18 •
2013
In pressure myography, an intact small segment of a vessel is mounted onto two small cannulas and pressurized to a suitable luminal pressure. Here, we describe the method to measure vasorelaxation response of the mouse 3rd order mesenteric arteries in c57 and sGCα1-/- mice using pressure myography.
11:02
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography
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Cited by 8 •
2012
Measurements of Kv7 (KCNQ) potassium channel activity in isolated arterial myocytes (using patch clamp electrophysiological techniques) in parallel with measurements of constrictor/dilator responses (using pressure myography) can reveal important information about the roles of Kv7 channels in vascular smooth muscle physiology and pharmacology.
09:05
Measurement of Smooth Muscle Function in the Isolated Tissue Bath-applications to Pharmacology Research
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Cited by 68 •
2015
This protocol describes the measurement of isometric contraction in an isolated smooth muscle preparation, using an isolated tissue bath system and computer-based data acquisition.
10:48
Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography
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Cited by 24 •
2015
Pressure myography is used to assess vasoactivity of small arteries that develop sustained constriction when pressurized. This manuscript provides a detailed protocol to assess in isolated segments of small mesenteric arteries from rats, vasoactivity and the effect of intraluminal pressure on vascular diameter.
07:25
Microinjection of Medaka Embryos for use as a Model Genetic Organism
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Cited by 26 •
2010
Medaka and zebrafish are complementary for genetic dissection of vertebrate genome functions. This protocol highlights the key points for successful microinjection into medaka embryos, an important technique for embryological and genetic analysis using medaka and zebrafish in a laboratory.
FAQs
A force or tension transducer detects changes produced when the mounted tissue contracts or relaxes. The recorded response provides a functional readout of tissue behavior rather than only a molecular measurement. Comparing force changes after different interventions can help characterize smooth muscle physiology, drug effects, receptor activity, and signaling pathways in isolated blood vessels or airways.
Agonists are applied to provoke a contractile or relaxant response, whereas antagonists help examine how that response depends on receptor activity. Observing changes in tissue tension after these treatments can indicate which receptor-related processes contribute to the response. This makes the technique useful for investigating pharmacological mechanisms and how candidate compounds influence smooth muscle function.
The isolated tissue is maintained in a temperature-controlled bath containing oxygenated physiological solution, creating standardized conditions for measurement. These conditions support tissue function while responses are recorded. Consistent control of the bathing environment helps researchers attribute changes in tension to the tested drug, agonist, antagonist, or stimulation rather than to avoidable changes in the experimental setting.
Electrical stimulation provides an experimental input that differs from directly applying a drug or receptor-directed compound. Recording the resulting change in force allows investigators to examine how the isolated tissue responds to controlled stimulation as well as pharmacological interventions. Using both approaches can broaden assessment of tissue function and help relate contractile behavior to physiological or signaling mechanisms.
Researchers first prepare and mount an isolated tissue segment, such as a blood vessel or airway, in the bath. The tissue is connected to a force or tension transducer while the surrounding solution is temperature controlled and oxygenated. Drugs, agonists, antagonists, or electrical stimulation are then applied, and the resulting contractile or relaxant changes are recorded.
The core setup includes an organ bath, physiological solution, temperature control, an oxygenation source, and a force or tension transducer. The tissue must remain mounted in the bath while interventions are delivered and tension is monitored. Together, these components provide the controlled environment and measurement system needed to assess responses from isolated smooth muscle preparations.
This approach supports studies of vascular reactivity, airway smooth muscle behavior, receptor activity, signaling pathways, and tissue function. It can also be used to examine drug effects and evaluate therapies relevant to cardiovascular and respiratory disorders. Because responses are measured directly in isolated tissues, the method connects pharmacological interventions with functional changes in medically relevant preparations.