Orthotopic Tumor
Orthotopic Tumor - Related Videos
06:23
A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
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Cited by 8 •
2017
This protocol describes the generation of murine orthotopic bladder tumors in female C57BL/6J mice and the monitoring of tumor growth.
03:59
Orthotopic Homograft Tumor Transplantation: A Technique to Generate Pancreatic Tumor Mouse Models
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2023
The video explains a stepwise surgical method for orthotopic implantation of a tumor fragment from a cancerous donor to the pancreas of a non-cancerous recipient mice for development of pancreatic cancer. Orthotopically implanted PDAC could recapture the tissue microenvironment of human pancreatic cancers than conventional subcutaneous (SC) models more accurately.
04:11
DCE-MRI of Orthotopic Pancreatic Tumor Xenografts: A Method to Assess Microvasculature in a Target Tumor Tissue
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2023
This video describes the technique of DCE-MRI for orthotropic pancreatic tumor xenografts in mice. DCE-MRI is a non-invasive method to analyze microvasculature in a target tissue, and useful to assess vascular response in a tumor following a novel therapy. This method will assist investigators to apply DCE-MRI for orthotopic gastrointestinal cancer mouse models.
08:43
An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment
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Cited by 12 •
2012
Establishing an orthotopic bladder tumor model to evaluate antitumor effects of intravesically delivered saRNA and monitoring tumor growth by ultrasound and bioluminescent imaging.
10:35
An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
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Cited by 18 •
2010
To study in vivo tumor growth and tumor microenvironment, we used a syngeneic and orthotopic mouse model of ovarian cancer in immunocompetent animals. We transduced a mouse tumor cell line (MOV1) with Katushka fluorescent protein (MOV1KAT) and here we show its orthotopic implantation in ovary and in vivo imaging.
FAQs
Local anatomy does more than provide a physical location: it brings the implanted cancer material into contact with stromal cells, blood vessels, extracellular matrix, and immune components. These interactions can modify tumor growth and spread, making the observed behavior more representative of how the disease functions within its native tissue environment.
Compared with an ectopic model, an orthotopic tumor model places the cancer in the anatomical setting where the corresponding malignancy arises. That distinction preserves local tissue interactions that may be absent or altered at a nonmatching site, so researchers can compare tumor growth, dissemination, and treatment responses under different modeling conditions.
Interactions with the native tissue environment make these models useful for examining invasion and metastasis, not only primary tumor expansion. Local stromal cells, vessels, extracellular matrix, and immune components can influence how disease progresses. Studying those relationships helps connect cancer-cell behavior with the surrounding biology when evaluating mechanisms of spread.
To establish the model, researchers introduce tumor cells, tissue fragments, or patient-derived material into the organ or tissue matching the cancer’s natural origin. The resulting model can then be used to examine tumor growth and spread, or to assess how treatment performs in that anatomically relevant setting.
Choice of implanted material allows the model to represent cancer in different ways. Researchers may use cultured tumor cells, pieces of tumor tissue, or material derived from a patient. These options support studies ranging from general cancer biology to behavior based on patient-derived material, while the matching anatomical site supplies the relevant tissue context.
Orthotopic tumor models are particularly valuable in preclinical drug evaluation because treatment outcomes may better reflect conditions in patients than results from a nonmatching site. They also support investigation of cancer biology, invasion, metastasis, and therapeutic response. Their main contribution is a test setting that combines drug assessment with anatomically relevant tumor behavior.