Orthotopic Xenograft Model
Orthotopic Xenograft Model - Related Videos
09:43
Primary Orthotopic Glioma Xenografts Recapitulate Infiltrative Growth and Isocitrate Dehydrogenase I Mutation
0 Views •
Cited by 10 •
2014
Malignant gliomas constitute a heterogeneous group of highly infiltrative glial neoplasms with distinct clinical and molecular features. Primary orthotopic xenografts recapitulate the histopathological and molecular features of malignant glioma subtypes in preclinical animal models.
07:44
Establishment of Orthotopic Patient-derived Xenograft Models for Brain Tumors using a Stereotaxic Device
0 Views •
2025
The development of orthotopic pediatric brain tumor models requires meticulous precision, using a stereotaxic device to implant cancer cells precisely. The methodology presented here outlines the steps involved in preparing brain tumor cells, performing intracranial injections, and implementing a post-operative monitoring system to assess brain tumor engraftment.
08:15
Minimally Invasive Establishment of Murine Orthotopic Bladder Xenografts
0 Views •
Cited by 16 •
2014
The established technique to inoculate primary invasive orthotopic bladder cancer xenografts requires laparotomy and mobilization of the bladder. This procedure inflicts significant morbidity on the mice, is technically challenging and time-consuming. We therefore developed a high-precision, percutaneous approach utilizing ultrasound guidance.
03:53
An Orthotopic Sciatic Nerve Xenograft for Neurofibromatosis Type 1 Neurofibromas
0 Views •
2025
Neurofibromatosis type 1 (NF1) leads to benign peripheral nerve sheath tumors (neurofibromas) that require a specific nerve microenvironment for growth. An optimized orthotopic xenograft model was developed, using intraneural injection of human Schwann cells into NSG mice. This model recapitulates human neurofibromas and provides a valuable platform to evaluate therapeutic strategies.
07:01
An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
0 Views •
Cited by 10 •
2013
Generation of an orthotopic mouse model of anaplastic thyroid carcinoma is described here. This technique employs surgical placement of human anaplastic thyroid cancer cells into the thyroid of immunodeficient mice, thus creating a more clinically relevant setting to study disease progression as well as screen innovative therapeutic interventions.
FAQs
The implanted human tumor encounters stromal signals, blood supply, and tissue architecture associated with its corresponding organ. These local features can alter how the tumor grows, invades nearby tissue, and spreads to other sites. As a result, the model can reveal disease behaviors that may not emerge when tumor material develops outside its usual anatomical environment.
The major distinction is anatomical context. Ectopic models place tumor material outside the organ in which the cancer normally develops, whereas orthotopic implantation preserves organ-specific surroundings. Because local stroma, vascular support, and tissue organization influence tumor behavior, the two approaches may differ in observed growth, invasion, metastasis, and response to treatment.
An immunodeficient animal provides the host setting used for implantation of human tumor cells or tissue. Within that setting, researchers can examine tumor progression in the selected organ and assess disease-related or treatment-related outcomes. The model therefore combines a human tumor source with an organ-relevant site for preclinical investigation.
This approach can be used to examine tumor progression, local invasion, and metastasis in relation to the organ environment. Because the graft develops amid tissue architecture and blood supply relevant to that site, researchers can study how anatomical context shapes these behaviors and use the resulting observations to investigate mechanisms of disease.
The essential workflow is to select human tumor cells or tissue, place the material into the corresponding organ of an immunodeficient animal, and then evaluate the developing cancer. Subsequent studies may focus on disease mechanisms, imaging strategies, or anticancer treatment effects. The specific organ and tumor material determine the biological context being examined.
Researchers may choose this model when treatment effects need to be assessed in an anatomically relevant tumor setting. It supports evaluation of tumor progression and drug response while preserving local stromal, vascular, and architectural influences. In medicine, those observations can contribute to preclinical therapeutic development and help connect laboratory findings with clinical research.
Imaging strategies can be evaluated in a model where the tumor develops within its corresponding organ rather than in an unrelated site. This context allows investigators to examine imaging in relation to tumor progression and the surrounding tissue environment. Such studies can support assessment of how effectively an approach characterizes disease in a biologically relevant setting.
Orthotopic xenografts contribute to translational medicine by placing preclinical observations in a context that more closely reflects organ-associated tumor behavior. Findings on disease mechanisms, imaging, progression, metastasis, or drug response can inform subsequent clinical research. The model therefore serves as an intermediate research system between laboratory experiments and development of cancer therapies.