Picco2 System
Picco2 System - Related Videos
09:09
Large-Scale Production of Extracellular Vesicles from High-Density 3D Cell Cultures in Hollow Fiber Perfusion Bioreactors
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2026
Instructions are provided for setting up and using a 3D perfusion hollow fiber bioreactor for extracellular vesicle production from MSC and other cell types. This protocol describes a method for producing extracellular vesicles at high concentrations on a continuous basis while controlling cell density in hollow fiber bioreactors.
11:08
Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles
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Cited by 15 •
2012
Lentiviral expression vectors are the most effective vehicles for stably expressing different effector molecules or reporter constructs in dividing and non-dividing mammalian cells and whole organisms. Here we provide a protocol on how to package lentivector expression constructs in pseudoviral particles and to transduce target cells using the pseudoviral particles.
07:03
In Situ Surface Temperature Measurement in a Conveyor Belt Furnace via Inline Infrared Thermography
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Cited by 3 •
2020
This protocol describes how to install an infrared camera into a conveyor belt furnace, conduct a customer correction of a factory calibrated IR camera, and evaluate the spatial surface temperature distribution of an object of interest. The example objects are industrial silicon solar cells.
09:06
The Effect of Construction and Demolition Waste Plastic Fractions on Wood-Polymer Composite Properties
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2020
Secondary material streams have been shown to include potential raw materials for production. Presented here is a protocol in which CDW-plastic waste as a raw material is identified, followed by various processing steps (agglomeration, extrusion). As a result, a composite material was produced, and mechanical properties were analyzed.
09:41
Functional Transcranial Doppler Ultrasound for Monitoring Cerebral Blood Flow
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Cited by 8 •
2021
Functional transcranial Doppler ultrasound complements other functional imaging modalities, with its high temporal resolution measurement of stimulus-induced changes in cerebral blood flow within the basal cerebral arteries. This Methods paper gives step-by-step instructions for using functional transcranial Doppler ultrasound to perform a functional imaging...
FAQs
The two methods provide complementary information. Transpulmonary thermodilution supplies intermittent measurements that calibrate the system, while pulse contour analysis uses the arterial pressure waveform to track cardiac performance continuously between calibrations. This combination helps clinicians follow changing hemodynamic conditions rather than relying only on occasional measurements, supporting more responsive assessment during critical illness.
A cold saline bolus enters through a central vein and travels through the cardiopulmonary circulation to a thermistor-tipped arterial catheter. The catheter detects the resulting temperature change, allowing the system to calculate cardiac output and related measurements. This indicator-based process also supports evaluation of preload, fluid responsiveness, and extravascular lung water.
The measurements help distinguish whether a critically ill patient may need additional fluid, closer assessment of cardiac function, or adjustment of vasoactive treatment. Preload and fluid-responsiveness data address circulatory filling, while extravascular lung water provides information about fluid outside the vessels in the lungs. Together, these variables support a more individualized cardiovascular assessment.
The monitoring process requires a central venous route for the cold saline bolus and an arterial catheter containing a thermistor. After the bolus travels to the arterial sensor, transpulmonary thermodilution generates calibration information for pulse contour analysis. The calibrated system can then provide ongoing cardiac performance and fluid-status measurements for clinical assessment.
Its use is relevant when patients require close circulatory monitoring, including those with shock, during complex surgery, or in other forms of critical illness. In these settings, clinicians can examine cardiac performance, preload, fluid responsiveness, and extravascular lung water together. The resulting profile can help guide individualized fluid therapy and vasoactive treatment.
Rather than treating circulation from a single measurement, clinicians can use the system's combined data to relate cardiac performance to fluid status. Evidence about preload and fluid responsiveness can inform fluid therapy, while cardiac-function information can support vasoactive treatment decisions. Extravascular lung-water measurements add context when evaluating the consequences of fluid management in critically ill patients.