Portable Gas Chromatograph

Portable gas chromatographs are compact analytical instruments that separate and identify volatile compounds in samples, bringing laboratory-style chemical analysis closer to clinical and field settings. They vaporize a sample and carry it through a coated capillary column with an inert gas; compounds interact differently with the stationary phase, so they elute at distinct retention times and are measured by a detector. In medicine, these systems can analyze breath metabolites, anesthetic gases, biological volatiles, and toxicants, supporting rapid screening and exposure assessment. Their small size and reduced analysis time may enable near-patient testing, emergency response, and monitoring where conventional laboratory instruments are impractical.

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JoVE Journal - Chemistry
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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

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Cited by 8 •

2020

This protocol presents an approach to fingerprint and explore multi-dimensional data collected by comprehensive two-dimensional gas chromatography coupled to mass spectrometry. Dedicated pattern recognition algorithms (template matching) are applied to explore the chemical information encrypted in the extra-virgin olive oil volatile fraction (i.e., volatilome).

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JoVE Core - Analytical Chemistry

Chromatographic Resolution

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2024

In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness. The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample. In chromatography,...

Chromatographic Methods: Terminology

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2024

Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...

Chromatographic Methods: Classification

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2024

Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface. Chromatographic techniques are typically named by...

Optimizing Chromatographic Separations

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2024

Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor. Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...

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