Radio-labeled Inulin
Radio-labeled Inulin - Related Videos
10:13
Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
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Cited by 11 •
2017
Guanosine triphosphate (GTP) binding is one of the earliest events in G-Protein-Coupled Receptor (GPCR) activation. This protocol describes how to pharmacologically characterize specific GPCR-ligand interactions by monitoring the binding of the radio-labeled GTP analog, [35S]guanosine-5'-O-(3-thio)triphosphate ([35S]GTPγS), in response to a ligand of interest.
09:29
The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies
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Cited by 81 •
2015
Due to its multi-day radioactive half-life and favorable decay properties, the positron-emitting radiometal 89Zr is extremely well-suited for use in antibody-based radiopharmaceuticals for PET imaging. In this protocol, the bioconjugation, radiosynthesis, and preclinical application of 89Zr-labeled antibodies will be described.
09:19
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
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Cited by 3 •
2025
This protocol presents a step-by-step guide for the Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopic technique, including details on how to generate the laser light source, prepare a tissue sample, conduct imaging, and analyze the data. SLAM advances nonlinear microscopy by measuring four complementary label-free contrasts to investigate the tissue microenvironment.
07:23
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
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Cited by 5 •
2016
A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
It prevents renal handling after filtration from distorting the measurement. Because the tracer is not added to or removed from tubular fluid, its clearance reflects the filtration activity of the glomeruli rather than a combination of filtration and tubular transport. This makes the result particularly useful when altered kidney performance must be quantified directly.
The label provides a measurable signal that can be tracked in blood or urine after administration. Quantifying that radioactivity allows investigators to determine how the tracer distributes and clears, producing a numerical assessment of filtration. The signal-based approach supports precise measurements that can be compared across evaluations of renal function.
Its renal handling provides a direct filtration-based benchmark, so measurements from newer or alternative biomarkers can be compared with an established estimate of glomerular filtration. This comparison helps investigators examine whether another approach accurately reflects kidney performance, especially when studying changes in filtration or validating a proposed measurement method.
The tracer is administered, then its radioactivity is measured in blood or urine. These measurements are used to follow inulin distribution and clearance, allowing glomerular filtration to be estimated quantitatively. The workflow can therefore provide a direct assessment of renal performance rather than relying only on indirect interpretation of a kidney biomarker.
The method is useful when precise evaluation of renal function is needed, including investigations of altered filtration. In medicine, it can support detailed assessment of kidney performance; in biomedical research, it can provide a quantitative basis for examining filtration and comparing results with other measures of renal status.
Researchers can use its quantitative filtration estimate as a reference for evaluating newer approaches. Comparing results allows investigators to determine how closely an alternative method reflects renal filtration and whether it remains informative when kidney function is altered. This role makes the technique important in method validation and comparative renal research.