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The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies
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JoVE Journal Chemistry
The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies

The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies

DOI:
10.3791/52521-v

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09:29 min

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February 12, 2015

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1Department of Radiology,Memorial Sloan Kettering Cancer Center

Chapters

  • 00:05Title
  • 01:15Conjugation of DFO-NCS to J591
  • 02:56Radiolabeling J591-DFO with 89Zr
  • 06:16In Vivo PET Imaging with 89Zr-DFO-J591
  • 07:52Results: PET Imaging with 89Zr-DFO-J591
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

Due to its multi-day radioactive half-life and favorable decay properties, the positron-emitting radiometal 89Zr is extremely well-suited for use in antibody-based radiopharmaceuticals for PET imaging. In this protocol, the bioconjugation, radiosynthesis, and preclinical application of 89Zr-labeled antibodies will be described.

Tags

Keywords: BioconjugationRadiosynthesis89ZrDFOAntibodiesPET ImagingRadiopharmaceuticalsTumor-targetingJ591 AntibodyProstate-specific Membrane Antigen

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