Cantilever Tip Scanning
Cantilever Tip Scanning - Related Videos
01:13
Impact Loading on a Cantilever Beam
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2024
The analysis of a cantilever beam with a circular cross-section subjected to impact loading at its free end illustrates the conversion of potential energy from a dropped object into kinetic energy, which is then absorbed by the beam as strain energy. This process is crucial for understanding how materials behave under dynamic loads, which is important in fields such as construction and aerospace. When an object is dropped onto the free end of a cantilever, its potential energy due to gravity is...
06:44
Cantilever Bending of Murine Femoral Necks
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2022
The present protocol describes the development of a reproducible testing platform for murine femoral necks in a cantilever bending set-up. Custom 3D printed guides were used to consistently and rigidly fix the femurs in optimal alignment.
02:28
Leaky Scanning
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2020
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
12:03
Differential Scanning Calorimetry
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2023
Source: Danielle N. Beatty and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Differential scanning calorimetry (DSC) is an important measurement to characterize thermal properties of materials. DSC is used primarily to calculate the amount of heat stored in a material as it heats up (heat capacity) as well as the heat absorbed or released during chemical reactions or phase changes. However, measurement of this heat can also lead to...
11:41
Scanning Electron Microscopy (SEM)
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2023
Source: Laboratory of Dr. Andrew J. Steckl — University of Cincinnati A scanning electron microscope, or SEM, is a powerful microscope that uses electrons to form an image. It allows for imaging of conductive samples at magnifications that cannot be achieved using traditional microscopes. Modern light microscopes can achieve a magnification of ~1,000X, while typical SEM can reach magnifications of more than 30,000X. Because the SEM doesn’t use light to create images, the resulting pictures it...
FAQs
The probe encounters changes in surface height and interaction forces as it moves across a sample. These encounters bend the flexible cantilever by different amounts, and an optical or electronic detector records the resulting deflection. The measured signal can then represent both surface topography and mechanical behavior, allowing researchers to relate nanoscale structural features to local physical properties.
Topography is inferred from changes in surface height, whereas mechanical information comes from the forces acting between the probe and the sample. Because these interactions affect cantilever bending, the same scan can provide complementary information about where structures are located and how they respond mechanically. This combination is useful when surface organization alone does not explain biological behavior.
Near-physiological conditions allow neuronal membranes, synapses, cytoskeletal structures, and related biomaterials to be examined in conditions that better preserve their biological context. This is especially relevant for studying structural organization and dynamic changes rather than only fixed or isolated features. The approach can also operate without fluorescent labels, reducing reliance on labeling for nanoscale surface measurements.
A sharp probe mounted on a flexible cantilever is positioned over the sample and moved across its surface. As the tip encounters height variations or interaction forces, the cantilever bends. An optical or electronic detector captures that deflection, and the recorded changes are used to construct a high-resolution map of surface topography and associated mechanical properties.
In neuroscience, measurements can target neuronal membranes, synapses, cytoskeletal structures, and biomaterials. Mapping these structures at the nanoscale can reveal their organization, stiffness, adhesion, and structural changes. The range of measurable features makes the technique relevant to questions about how neuronal components are arranged and how their physical characteristics change during biological processes.
The method supports investigations of neuronal development, signaling, and neurodegenerative disease by linking nanoscale structure with physical properties. Researchers can assess organization, stiffness, adhesion, and dynamic structural changes in relevant neural samples or biomaterials. These measurements provide a way to examine how altered nanoscale architecture or mechanics may accompany developmental, signaling-related, or disease-associated changes.