Di-8-aneppq Dye
Di-8-aneppq Dye - Related Videos
05:52
Measuring the Induced Membrane Voltage with Di-8-ANEPPS
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Cited by 48 •
2009
External electric field induces a voltage on the membrane of a cell, termed the induced membrane voltage (ΔΦ). By using the potentiometric dye di-8-ANEPPS, it is possible to measure the ΔΦ noninvasively. This video shows the protocol for measuring ΔΦ using di-8-ANEPPS.
03:15
Screening of Cyclic di-GMP Modulators Using Bacteria Expressing a Fluorescent Reporter Protein
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2025
Source: Rugjee, K. N., et al. Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa. J. Vis. Exp. (2016).This video demonstrates the screening of cyclic di-GMP modulators using genetically modified bacteria that express green fluorescent protein (GFP) under the control of a c-di-GMP-sensitive promoter. Fluorescence is measured in a multi-well plate containing different test compounds. Compounds that inhibit c-di-GMP...
10:30
Dye-sensitized Solar Cells
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2023
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University Today's modern world requires the use of a large amount of energy. While we harness energy from fossil fuels such as coal and oil, these sources are nonrenewable and thus the supply is limited. To maintain our global lifestyle, we must extract energy from renewable sources. The most promising renewable source, in terms of abundance, is the sun, which provides us with more than enough solar energy to fully fuel our...
08:36
FM Dyes in Vesicle Recycling
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2023
FM dyes are a class of fluorescent molecules that has found important use in studying the vesicle recycling process. By virtue of a chemical structure, these molecules can insert themselves into the outer leaflet of phospholipid bilayer membranes. After membrane insertion, they are internalized into the cell via endocytosed vesicles, and released when these vesicles recycle back to the membrane. Since, these dyes fluoresce strongly in the hydrophobic environment within membranes and weakly in...
11:31
Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
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Cited by 2 •
2016
This article describes the high-throughput assay that has been successfully established to screen large libraries of small molecules for their potential ability to manipulate cellular levels of cyclic di-GMP in Pseudomonas aeruginosa, providing a new powerful tool for antibacterial drug discovery and compound testing.
FAQs
The dye's styryl chromophore undergoes an electrochromic shift in response to the electric field across the membrane. Because Di-8-ANEPPS partitions into the lipid bilayer, changes in transmembrane potential alter its fluorescence rapidly. This converts electrical changes associated with neuronal activity into an optical signal that can be monitored in living preparations.
Localization within the lipid bilayer places the voltage-sensitive chromophore where the membrane electric field acts. This positioning links fluorescence changes to transmembrane potential rather than treating the dye as a general indicator throughout the cell. In neural tissue, that relationship supports optical tracking of membrane excitability and electrical signal propagation.
As neurons depolarize or repolarize, the transmembrane potential changes in opposite directions. Di-8-ANEPPS responds through its voltage-dependent electrochromic shift, so the resulting fluorescence dynamics follow these electrical transitions. The rapid optical response is particularly relevant for examining action-potential-related activity, where the timing of membrane changes is central to interpreting neuronal function.
Optical sensing provides a spatially distributed view of voltage-related activity, whereas electrophysiological recordings can be difficult or spatially limited in some preparations. Di-8-ANEPPS therefore helps connect membrane-level electrical events with signal propagation across neural tissue. It offers a complementary optical readout when researchers need broader cellular or circuit-level context.
A basic use sequence is to introduce the dye into living cells or neural tissue, allow it to partition into the lipid bilayer, and monitor fluorescence while membrane potential changes. The recorded optical signal can then be related to depolarization, repolarization, action-potential-related activity, or propagation across the preparation.
Measurements can target several scales of neural function: action-potential-related activity at the membrane, membrane excitability at the cellular level, and signal propagation through neural tissue. Because the readout is voltage dependent, fluorescence changes provide an optical correlate of electrical dynamics and help relate individual neuronal events to broader activity patterns.
It is especially useful when direct electrophysiological recording is difficult or offers limited spatial coverage. In those situations, fluorescence-based voltage measurements can reveal electrical activity and how signals move through living neural tissue. This makes the dye relevant for linking neuronal function to cellular and circuit-level dynamics.