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Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
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Chapters
- 00:05Title
- 00:59Generation of GFP Tagged c-di-GMP Reporter P. aeruginosa Strain
- 04:21Preparation of the Starter Culture for Inoculation
- 05:35Inoculation and Incubation of the 384-well Plates Containing Small Molecules
- 06:33Measurement and Analysis of Growth and Intracellular c-di-GMP Level
- 08:25Results: Hits Obtained Following the High-throughput Screen against P. aeruginosa
- 10:07Conclusion
This article describes the high-throughput assay that has been successfully established to screen large libraries of small molecules for their potential ability to manipulate cellular levels of cyclic di-GMP in Pseudomonas aeruginosa, providing a new powerful tool for antibacterial drug discovery and compound testing.
