Fluorescent Antibody Tracking
Fluorescent Antibody Tracking - Related Videos
08:39
Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy
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2025
This article provides a detailed description of how to create samples for single-protein tracking in solid-supported lipid bilayers. It also explains a straightforward fluorescence microscope with single-molecule sensitivity and a fast frame rate. Finally, we outline the procedure for extracting single-protein trajectories.
11:27
Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
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Cited by 2 •
2008
We describe a reliable method for immunolocalization within the epidermis modified for both frozen and paraffin sections that we use very routinely in our laboratory.
06:48
Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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Cited by 3 •
2016
The on-bead method for labeling antibodies with small molecules enables labeling of a small amount of antibodies directly from cell media. This method is compatible with amine and thiol chemistry, and can handle multiple samples in parallel, manually or using automated platforms.
03:49
A Luciferase-Fluorescent Reporter Influenza Virus to Track Viral Infections
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2025
This video demonstrates a method to track viral infections in mouse models using a recombinant luciferase and fluorescence protein-expressing bi-reporter Influenza A virus. This method enables both in vivo and ex vivo studies, enabling researchers to gain insights into viral dynamics and the effects of interventions.
02:44
An In Vivo Technique to Track Bispecific Antibody-Induced T Cell Trafficking to Tumors
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2025
This video demonstrates a method for tracking bispecific antibody-induced T cell trafficking to tumors in a mouse model. Luciferase-transduced effector T cells are injected into a mouse with a subcutaneous tumor cell xenograft. Bispecific antibodies are then introduced, binding to T cells and the tumor cells. The localization of the T cells is tracked using bioluminescent imaging, with the magnitude of the signal indicating the extent of T cell infiltration into the tumor.
FAQs
Selectivity comes from antibody-antigen recognition: the antibody binds a particular molecular target instead of labeling all components of the sample equally. The attached fluorophore then provides the detectable signal after excitation. This combination links molecular specificity with optical readout, allowing investigators to associate fluorescence with the location of a chosen protein, cell, or other antigen.
A fluorophore absorbs light at an excitation wavelength and emits light at characteristic wavelengths that can be detected separately from the incoming illumination. These optical properties make the antibody-bound target visible to fluorescence microscopy or related instruments. The resulting signal supports localization of the antigen and comparison of its distribution across cells, tissues, or experimental conditions.
In neuroscience, the approach can identify neurotransmitter receptors, neuronal markers, synaptic proteins, and disease-associated molecules. Each target provides a different biological view: receptors relate to signaling, neuronal markers help identify cellular populations, synaptic proteins indicate connection-related structures, and disease-associated molecules support investigation of pathological changes. Together, these readouts connect molecular distribution with neural organization and function.
The core components are a biological sample, an antibody that recognizes the selected antigen, and a fluorescent label attached to the antibody. Detection requires fluorescence microscopy or another related instrument capable of exciting the fluorophore and recording its emitted light. These elements work together to convert selective molecular binding into an observable spatial pattern within the sample.
Fluorescent antibody tracking enables investigators to compare where a target appears and how its distribution changes over time or under different experimental conditions. Such comparisons can reveal shifts in receptor, synaptic protein, neuronal marker, or disease-associated molecule patterns. The value lies in relating changes in fluorescence location or distribution to developmental, signaling, connectivity, or disease-related questions.
The technique helps map molecular features involved in neural development, connectivity, signaling, and neurological disease mechanisms. Researchers can examine the distribution of selected targets within cells and tissue, then assess how those patterns differ among relevant conditions. This provides a spatial molecular perspective that complements broader studies of neuronal organization and disease-associated changes.