Grin Lens Coating

GRIN lens coating is a thin optical layer applied to a gradient-index (GRIN) lens to control surface reflection and light transmission, an important consideration in compact imaging systems used in neuroscience. The coating typically uses one or more carefully selected thin films whose refractive indices and thicknesses produce destructive interference for reflected wavelengths, increasing transmission while preserving image quality; designs may be optimized for excitation and emission light in fluorescence imaging. By improving optical efficiency and contrast, GRIN lens coatings can support miniature endoscopes and implanted imaging assemblies used to visualize neural structure or activity in otherwise inaccessible brain regions.

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Research

JoVE EoE - Danio rerio (zebrafish)

Eye Lens Dissection: A Technique to Observe Zebrafish Lens Morphology

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2023

This video describes the protocol which is developed to dissect adult and larval zebrafish lenses to analyze cortical lens morphology and structural integrity of the lens sutures.

Simulating the Mechanics of Lens Accommodation via a Manual Lens Stretcher

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Cited by 9 •

2018

We present an efficient method of studying lens accommodation by using a manual lens stretcher. The protocol mimics physiological accommodation by pulling the zonules connected around the lens capsule, thereby, stretching the lens.

Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants

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Cited by 4 •

2009

Lens development involves interactions with other tissues. Several zebrafish eye mutants are characterized by an abnormally small lens size. Here we demonstrate a lens transplantation experiment to determine whether this phenotype is due to intrinsic causes or defective interactions with tissues that surround the lens.

Research

JoVE Journal - Biology
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A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

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Cited by 7 •

2011

In newt, the lens regenerates always from the dorsal iris by transdifferentiation of the iris pigmented epithelial cells (IPEs). Here we describe a procedure to culture dorsal and ventral newt IPE cells and their implantation to the newt eye. The implanted cells are then studied by tissue sectioning and immunohistochemistry.

Graphene Coatings for Biomedical Implants

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Cited by 37 •

2013

Graphene offers potential as a coating material for biomedical implants. In this study we demonstrate a method for coating nitinol alloys with nanometer thick layers of graphene and determine how graphene may influence implant response.

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