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A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
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A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

Article DOI: 10.3791/2713-v • 06:57 min • June 22nd, 2011
June 22nd, 2011 •
*1, *1, 1,2
1Department of Biology, University of Dayton, 2Center for Tissue Regeneration and Engineering, University of Dayton
* These authors contributed equally

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Summary
Automatic Translation

In newt, the lens regenerates always from the dorsal iris by transdifferentiation of the iris pigmented epithelial cells (IPEs). Here we describe a procedure to culture dorsal and ventral newt IPE cells and their implantation to the newt eye. The implanted cells are then studied by tissue sectioning and immunohistochemistry.

Tags

Iris Pigment Epithelial Cells Lens Regeneration Newt Salamanders Axolotl Regenerative Capability Transdifferentiation Dorsal Iris Cells Ventral Iris Cells In Vitro Culture Transplantation Reaggregation Gene And Protein Study
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