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JoVE Journal
Biology

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A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
 

A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

Article DOI: 10.3791/2713
June 22nd, 2011

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Summary June 22nd, 2011

In newt, the lens regenerates always from the dorsal iris by transdifferentiation of the iris pigmented epithelial cells (IPEs). Here we describe a procedure to culture dorsal and ventral newt IPE cells and their implantation to the newt eye. The implanted cells are then studied by tissue sectioning and immunohistochemistry.

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