Hydrogel Perfusion
Hydrogel Perfusion - Related Videos
07:25
Collagen Hydrogels
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2023
Collagen is another widely used biomaterial that has found popularity in commercial applications, such as photography. Collagen has more recently been used in tissue engineering applications, by creating hydrogels that provide structure to engineered tissue. This video introduces collagen as a biomaterial, demonstrates how it is harvested from porcine skin, and shows how the material is used to create a hydrogel for tissue engineering applications. Finally, several applications of the material...
08:33
Hydrogel Synthesis
0 Views •
2023
Source: Amber N. Barron, Ashlea Patterson, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Hydrogels are a versatile class of cross-linked polymers produced through relatively simple procedures and with generally inexpensive materials. They can be formed from solution and involve a polymer backbone formed from monomer reagents, an initiator which makes the polymer reactive and a crosslinking species which binds the polymer chains...
10:54
Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture
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Cited by 33 •
2017
This is a method to create a 3-dimensional cell culture scaffold from pulmonary extracellular matrix. Intact lung is processed into hydrogels that can support the growth of cells in three-dimensions.
09:47
FRET Imaging in Three-dimensional Hydrogels
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Cited by 3 •
2016
Förster resonance energy transfer (FRET) imaging is a powerful tool for real-time cell biology studies. Here a method for FRET imaging cells in physiologic three-dimensional (3D) hydrogel microenvironments using conventional epifluorescence microscopy is presented. An analysis for ratiometric FRET probes that yields linear ratios over the activation range is described.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
FAQs
Its main advantage is that flowing medium reaches more of the hydrogel than diffusion alone can support. As fluid passes through interconnected pores, it supplies nutrients and oxygen while carrying away metabolic waste. This can help sustain cells and engineered neural tissues within three-dimensional culture models.
Compared with static culture, Hydrogel Perfusion adds a continuous transport pathway through or around the polymer network. That distinction matters when cells or tissues are embedded in three-dimensional environments where diffusion alone may not adequately sustain them. The resulting access to nutrients, oxygen, and waste removal can make these models more consistent and physiologically relevant.
Fluid movement provides more than chemical exchange: it can expose cells to controlled mechanical cues generated by the moving medium. In neural cultures, this adds a controllable physical dimension to the cellular microenvironment alongside nutrient delivery and waste removal. That is useful for examining how engineered neural tissues respond within three-dimensional models.
A basic workflow begins with a water-rich, three-dimensional polymer network containing the cells or engineered tissue. Culture medium is then moved continuously through or around that network, allowing fluid access to its interconnected pores. Maintaining this arrangement supports transport and exposes the construct to the intended culture environment.
Researchers can use Hydrogel Perfusion when a neural culture, brain-like tissue model, or engineered neural construct is difficult to maintain by diffusion alone. The approach is especially relevant to experiments requiring three-dimensional neural environments for studying development, injury, disease, or tissue repair. It also supports more consistent experimental conditions.
In neuroscience, perfused hydrogel models can provide a setting for studying neural cells and tissues under improved nutrient and oxygen transport, waste removal, and controlled mechanical stimulation. These features help researchers investigate how brain-like or engineered neural constructs develop and respond to injury or disease, while also supporting studies of tissue repair.