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JoVE Journal
Biology

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FRET Imaging in Three-dimensional Hydrogels
 

FRET Imaging in Three-dimensional Hydrogels

Article doi: 10.3791/54135
August 1st, 2016

Summary August 1st, 2016

Förster resonance energy transfer (FRET) imaging is a powerful tool for real-time cell biology studies. Here a method for FRET imaging cells in physiologic three-dimensional (3D) hydrogel microenvironments using conventional epifluorescence microscopy is presented. An analysis for ratiometric FRET probes that yields linear ratios over the activation range is described.

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