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FRET Imaging in Three-dimensional Hydrogels

FRET Imaging in Three-dimensional Hydrogels

Article DOI: 10.3791/54135-v 09:47 min August 1st, 2016
August 1st, 2016



Förster resonance energy transfer (FRET) imaging is a powerful tool for real-time cell biology studies. Here a method for FRET imaging cells in physiologic three-dimensional (3D) hydrogel microenvironments using conventional epifluorescence microscopy is presented. An analysis for ratiometric FRET probes that yields linear ratios over the activation range is described.


FRET Imaging Three-dimensional Hydrogels Intracellular Signaling Developmental Biology Pharmacology Regenerative Medicine Biomaterials Drug Screening Wide Field Microscope Systems Hydrogel Casting Cultured Cells Crosslinking UVA Light Source Dental Lamp Cell Viability PDMS Hydrogel
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