Macroscopic Imaging
Macroscopic Imaging - Related Videos
08:09
A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
0 Views •
2014
A simple, robust and scalable technique to functionalize and self-assemble macroscopic nanoparticle-ligand monolayer films onto template-free substrates is described in this protocol.
07:56
Preparation of Liquid Crystal Networks for Macroscopic Oscillatory Motion Induced by Light
0 Views •
Cited by 11 •
2017
The goal of the protocol is to create liquid crystalline polymer films that can mechanically oscillate under continuous light irradiation. We describe in great detail the conception of free-standing films, from the liquid crystal alignment method to the photo-actuation. The experimental protocol applied to prepare this material is broadly applicable.
11:03
An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids
0 Views •
Cited by 2 •
2017
An experimentally accessible analog method for studying molecular hydrodynamic processes in dense fluids is presented. The technique uses particle image velocimetry of vibrated, high-restitution grain piles and allows direct, macroscopic observation of dynamical processes known and predicted to exist in strongly interacting, high density gases and liquids.
10:35
Using Microwave and Macroscopic Samples of Dielectric Solids to Study the Photonic Properties of Disordered Photonic Bandgap Materials
0 Views •
2014
Disordered structures offer new mechanisms for forming photonic bandgaps and unprecedented freedom in functional-defect designs. To circumvent the computational challenges of disordered systems, we construct modular macroscopic samples of the new class of PBG materials and use microwaves to characterize their scale-invariant photonic properties, in an easy and inexpensive manner.
02:41
In Vivo Imaging of Cerebrospinal Fluid Transport in a Mouse Brain Using Fluorescence Macroscopy
0 Views •
2025
Source: Sweeney, A. M., et al. In Vivo Imaging of Cerebrospinal Fluid Transport through the Intact Mouse Skull using Fluorescence Macroscopy. J. Vis. Exp. (2019)This video demonstrates the process of visualizing cerebrospinal fluid (CSF) flow in a mouse brain using fluorescence imaging. A fluorescent tracer is injected into the cisterna magna (CM), allowing its movement with CSF to be tracked through the subarachnoid and perivascular spaces. Time-lapse imaging captures the tracer's journey,...
FAQs
The measured signal shapes the biological interpretation. Cameras may detect emitted light, whereas scanners can measure changes in blood oxygenation or other signals. These differences determine whether the resulting maps emphasize activity, tissue organization, or changing physiological states. Selecting the signal therefore connects the imaging readout to the specific brain process under investigation.
Macroscopic Imaging captures patterns across large tissue areas, allowing researchers to examine relationships among regions rather than isolated cellular events. This scale can expose brain-wide organization, functional specialization, and coordinated dynamics that cellular-scale techniques may miss. The resulting observations are therefore especially useful for connecting local neural events with broader patterns of brain function.
A spatial map shows where a signal is distributed, while repeated measurements reveal how that distribution changes. Tracking signals over time helps researchers examine dynamic brain activity and relate local events to larger-scale responses. This temporal dimension can distinguish stable organizational features from activity patterns that emerge during sensory processing or other changing neural states.
Researchers first select an imaging modality that detects a relevant signal, then acquire data from the intact brain or exposed tissue using a camera or scanner. The detected signal is converted into spatial maps, often followed across time. Investigators can then examine broad activity patterns, connectivity, or functional specialization in relation to the experimental question.
Researchers may choose Macroscopic Imaging when the question concerns organization across large brain areas, coordinated activity, or relationships among regions. Cellular-scale methods provide finer local detail, but they may not capture brain-wide patterns as directly. Large-area imaging is therefore suited to linking local neural events with distributed dynamics and identifying functional specialization across tissue.
For sensory-processing studies, large-area maps can show how responses are distributed across the brain and how they change over time. In disease research, repeated or comparative imaging can monitor alterations in broad organization or activity patterns. These applications help relate localized neural events to brain-wide function and identify changes associated with neurological conditions.