Mito Turbo Red Protein
Mito Turbo Red Protein - Related Videos
13:42
Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
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Cited by 19 •
2012
A spectrofluorometric protocol for the measurement of the mitochondrial permeability transition pore opening in isolated mouse heart mitochondria is presented here. The assay involves the simultaneous measurement of mitochondria Ca2+ handling, mitochondrial membrane potential and mitochondrial volume. The procedure for obtaining high-quality and functional heart mitochondria is also described.
01:19
Translocation of Proteins into the Mitochondria
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2023
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases. Sorting of outer membrane proteins: Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
07:02
TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
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Cited by 37 •
2020
Described here is a proximity labeling method for identification of interaction partners of the TIR domain of the NLR immune receptor in Nicotiana benthamiana leaf tissue. Also provided is a detailed protocol for the identification of interactions between other proteins of interest using this technique in Nicotiana and other plant species.
10:39
Purification of Mitochondria from Yeast Cells
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Cited by 85 •
2009
We describe a rapid and effective method for purification of mitochondria from the yeast Saccharomyces cerevisiae. This method enables the high-yield isolation of pure mitochondria that are essentially free of contamination by other organelles and retain their structural and functional integrity after their purification.
05:37
Visualization of Mitochondria within Intraepidermal Nerve Fibers
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2025
Source: Hamid, H. S., et al. Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers. J. Vis. Exp. (2017) This video demonstrates the method for visualizing nerve-specific mitochondria using three-dimensional imaging. It outlines the process of acquiring sequential scans of pre-labeled intraepidermal nerve fibers and generating 3D surface images to isolate and display mitochondria in relation to nerve structures.
FAQs
The targeting sequence acts as the localization signal for the expressed protein. Once produced, it directs the reporter toward mitochondria rather than leaving the signal distributed generally throughout the cell. This targeting is important because it links the red fluorescence to mitochondrial position, allowing organization and behavior to be examined within living cells.
The chromophore is the light-responsive part of Mito Turbo Red protein. After excitation, it produces a red fluorescent signal that makes the targeted mitochondria visible. Because the signal can be observed repeatedly, researchers can use it to follow mitochondrial appearance and behavior over time rather than relying only on a static observation.
In neuronal cells, the reporter can reveal mitochondrial distribution, morphology, transport, and broader dynamics. These observations help researchers examine where mitochondria are positioned, how their structures appear, and how they behave within neurons and neuronal processes. Studying these features together provides a visual basis for relating mitochondrial behavior to the needs of neural cells.
Researchers express the genetically encoded reporter in living cells, allow its mitochondrial targeting sequence to direct the protein, and excite the resulting chromophore to generate red signal. Imaging over time then provides observations of mitochondrial organization and movement. This workflow supports comparisons of mitochondrial dynamics across neuronal processes or changing cellular conditions.
Mitochondrial labeling makes it possible to observe the distribution and movement of these organelles within neuronal processes, including axons. Such imaging helps researchers relate mitochondrial transport to the energy demands of neurons. The resulting view connects organelle behavior with how long neuronal extensions receive and organize mitochondria where cellular activity requires them.
Time-dependent fluorescence allows researchers to examine whether mitochondrial distribution, morphology, transport, or dynamics change when neurons experience injury or disease-related conditions. These visual measurements can help connect altered mitochondrial behavior with neuronal responses. In neuroscience, that connection provides context for understanding how mitochondrial organization and trafficking relate to cellular stress and dysfunction.