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JoVE Journal
Immunology and Infection

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TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
 

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

Article doi: 10.3791/60728
May 17th, 2020 Usage Statistics

Summary May 17th, 2020

Described here is a proximity labeling method for identification of interaction partners of the TIR domain of the NLR immune receptor in Nicotiana benthamiana leaf tissue. Also provided is a detailed protocol for the identification of interactions between other proteins of interest using this technique in Nicotiana and other plant species.

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