Mouse Neurosurgery
Mouse Neurosurgery - Related Videos
08:08
Simulator Training for Endovascular Neurosurgery
0 Views •
Cited by 3 •
2020
Simulation of complex, high-risk procedures is critical to the education of medical trainees. A protocol for simulator-based endovascular neurosurgery training in a controlled academic environment is described. The protocol includes stepwise guidelines for trainees of varying levels, with a discussion of the advantages and limitations of this model.
14:15
Multicolor 3D Printing of Complex Intracranial Tumors in Neurosurgery
0 Views •
Cited by 21 •
2020
The protocol describes the fabrication of fully colored three-dimensional prints of patient-specific, anatomical skull models to be used for surgical simulation. The crucial steps of combining different imaging modalities, image segmentation, three-dimensional model extraction, and production of the prints are explained.
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
0 Views •
Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
07:04
A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse
0 Views •
Cited by 5 •
2017
Mice are widely used to study gestational biology. However, pregnancy termination is required for such studies which precludes longitudinal investigations and necessitates the use of large numbers of animals. Therefore, we describe a non-invasive technique of high-frequency ultrasonography for early detection and monitoring of post-implantation events in the pregnant mouse.
07:46
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
0 Views •
Cited by 39 •
2010
Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.
FAQs
These components address different experimental needs. Anesthesia supports the surgical procedure, aseptic technique helps control contamination, and stereotaxic positioning provides a stable, precise orientation for work on defined brain regions. Using them together improves the consistency of access and manipulation, allowing researchers to relate a planned neural intervention more reliably to later physiological or behavioral measurements.
A small craniotomy creates access to the brain while limiting the extent of surgical disruption. Through this opening, researchers can deliver substances, place devices, or create targeted lesions. The specific intervention determines what neural feature is altered or monitored, helping experiments connect a defined manipulation with changes in neural function, behavior, or disease-related outcomes.
Closure and recovery help limit injury and other surgical effects that could confound interpretation. If postoperative consequences are not carefully controlled, measured physiological or behavioral changes may reflect the procedure rather than the intended brain manipulation. Attention to these stages therefore supports cleaner comparisons between manipulated animals and the outcomes used to evaluate neural circuits, disease mechanisms, or therapies.
A typical workflow combines anesthesia, aseptic preparation, precise placement in a stereotaxic apparatus, and a small craniotomy. Researchers then deliver a substance, place a device, or create a targeted lesion, followed by careful closure and recovery. This sequence organizes access, intervention, and stabilization so subsequent measurements can be interpreted in relation to the intended brain target.
Researchers may use these procedures when a study requires direct access to the mouse brain to alter or monitor neural systems. The approach supports investigations of neural circuits, behavior, disease mechanisms, and therapeutic strategies. Its value comes from pairing a defined anatomical or functional manipulation with physiological or behavioral outcomes, rather than observing those outcomes without an intervention.
Mouse neurosurgery can link brain interventions with physiological or behavioral outcomes. Those measurements may help researchers examine how neural circuits function, how disease mechanisms affect the brain, or whether a therapeutic strategy produces a measurable effect. Interpretation depends on relating the observed outcome to the specific substance delivery, device placement, or targeted lesion performed during the experiment.