Organ Chip Culture
Organ Chip Culture - Related Videos
01:35
Organ-on-Chip Models
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2026
Introduction to Organ-on-Chip Models Organ-on-chip models are advanced microphysiological systems that recreate key structural and functional features of human organs in a controlled laboratory setting. These devices integrate living human cells within microfluidic channels, allowing researchers to simulate tissue–tissue interfaces, fluid flow, and mechanical forces that are essential for normal organ function. Unlike traditional cell cultures, which often lack these dynamic physiological cues,...
06:12
Modeling the Endothelial Glycocalyx Post-Pneumonectomy in a 3D Fluidic Chip - An Approach to Fabricating a Vascular-based Organ-on-Chip System
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2025
Here we present a protocol for constructing an organ-on-chip (OOC) system designed to mimic pneumonectomy. This system investigates the potential correlation between glycocalyx impairment and complications following pneumonectomy.
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
10:36
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
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Cited by 6 •
2012
We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.
12:39
Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
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Cited by 7 •
2008
We describe a chip-based platform for the three-dimensional cultivation of cells in micro-bioreactors. One chip can house up to 10 Mio. cells that can be cultivated under precisely defined conditions with regard to fluid flow, oxygen tension etc. in a sterile, closed circulation loop.
FAQs
Microfluidic channels shape the culture environment by controlling nutrient delivery, fluid flow, and contact between different cell populations. These conditions help maintain tissue-specific interfaces rather than exposing every cell to an identical static medium. As a result, researchers can examine how neural, glial, and vascular cells behave together under more organ-like conditions.
Compared with conventional two-dimensional culture, organ chip culture can reproduce fluid movement and defined cell-to-cell interfaces within a small device. The improvement is not simply a change in container size: controlled microenvironments allow tissue behavior to be studied under conditions that more closely reflect organ structure and function. This supports more controlled, human-relevant experiments.
In neuroscience applications, the choice and arrangement of cells determines which biological interaction the model can address. A chip may bring neurons, glial cells, and vascular cells into a controlled relationship, or focus on the blood-brain barrier or neural tissue. That organization enables investigation of communication and tissue responses relevant to brain development and neurological disease.
A basic workflow begins by introducing living human cells into the device, establishing the intended tissue or organ model, and using the microfluidic channels to regulate nutrient delivery and flow. Researchers then examine the resulting tissue behavior or responses under controlled conditions. The exact setup depends on whether the target is neural tissue, the blood-brain barrier, or a cell interaction.
Drug transport and neurotoxicity are important applications because the device can place test compounds in a controlled tissue context. Brain-oriented chips can be used to study how substances interact with the blood-brain barrier or neural tissue and to evaluate responses involving neurons, glial cells, and vascular cells. These experiments connect exposure conditions with human-relevant neural outcomes.
Organ chip culture is especially useful when researchers need a controlled human-based complement to conventional models. In neuroscience, it supports studies of brain development, neurological disease, drug transport, and neurotoxicity while reducing reliance on animal models. Its value comes from combining human cells with regulated microenvironments, allowing specific variables and cell interactions to be examined more directly.