Teer Measurement Protocol
Teer Measurement Protocol - Related Videos
02:17
Measuring TEER in X-ray-Irradiated Epithelial Cells Co-cultured With PBMCs
0 Views •
2025
This video demonstrates the measurement of trans-epithelial electrical resistance in irradiated Caco-2 cells, both in the presence and absence of PBMC cells. This method enables the study of the synergistic effects of radiation and immune cells on the epithelial cell barrier.
07:43
A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency
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Cited by 15 •
2019
Here, we demonstrate how to set up an inexpensive volt-amperemeter with programmable output frequency that can be used with commercially available chopstick electrodes for transepithelial/endothelial electrical resistance measurements.
07:28
A Protocol of Manual Tests to Measure Sensation and Pain in Humans
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Cited by 24 •
2016
The goal of this procedure is to demonstrate a battery of quantitative techniques for sensory and pain measurement in humans. The equipment and techniques described are commonly found in pain clinics or are easy to obtain.
07:51
A Protocol for Measuring Cue Reactivity in a Rat Model of Cocaine Use Disorder
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Cited by 6 •
2018
Cue reactivity is conceptualized as sensitivity to cues linked with drug-taking experiences that contribute to craving and relapse in abstinent humans. Cue reactivity is modeled in rats by measuring attentional orientation toward drug-associated cues that results in appetitive approach behavior in a cue reactivity test following self-administration and forced abstinence.
10:56
A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
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Cited by 32 •
2014
A simple protocol to determine the neutral lipid content of algal cells using a Nile Red staining procedure is described. This time-saving technique offers an alternative to traditional gravimetric-based lipid quantification protocols. It has been designed for the specific application of monitoring bioprocess performance.
FAQs
Changes in TEER primarily reflect altered ionic passage through the cell layer. When intercellular junctions become tighter, fewer ions cross the barrier and measured resistance tends to increase. Conversely, reduced resistance is consistent with greater ionic permeability. The result provides an electrical readout of barrier integrity rather than a direct image of junction structure.
Electrode placement is essential because the electrical signal must pass across the cultured monolayer. Positioning electrodes on opposite sides allows the instrument to detect resistance associated with the barrier separating those compartments. This arrangement makes the reading relevant to the monolayer’s contribution to electrical resistance and supports interpretation of its integrity.
Because the assay is noninvasive, researchers can use electrical resistance as a readout while preserving the cultured layer for study. Measurements can support characterization of how an endothelial model develops a barrier and reveal changes when integrity is disrupted. This makes the approach useful for following the state of an in vitro neurovascular model.
A basic setup requires a cultured cell monolayer that forms an electrical barrier, an instrument that applies a small electrical signal, and electrodes placed on opposite sides of the layer. The protocol then records the resulting resistance. In neuroscience experiments, the monolayer may represent an in vitro endothelial blood-brain barrier model.
Researchers can compare resistance measurements from an endothelial barrier model before and after exposure to a treatment. An increase generally supports tighter junctional integrity and reduced ionic passage, whereas a decrease indicates greater permeability. This comparison helps determine whether an intervention appears to strengthen or disrupt the modeled blood-brain barrier.
TEER supplies a quantitative electrical indicator of barrier state in an endothelial monolayer. That indicator can be used to assess barrier formation, integrity, and disruption within a blood-brain barrier model. In neuroscience, the result helps connect cell-layer behavior with questions about neurovascular permeability and treatments or conditions that alter the barrier.